Target：GNL3

Fields：Ribosome biogenesis in eukaryotes

Gene Name：GNL3 E2IG3 NS

Protein Name：Guanine nucleotide-binding protein-like 3 (E2-induced gene 3 protein) (Novel nucleolar protein 47) (NNP47) (Nucleolar GTP-binding protein 3) (Nucleostemin)

Human Gene Id：26354

Human Swiss Prot No：Q9BVP2

Mouse Swiss Prot No：Q8CI11

Rat Swiss Prot No：Q811S9

Immunogen：Synthesized peptide derived from part region of human protein

Specificity：GNL3 Polyclonal Antibody detects endogenous levels of protein.

Formulation：Liquid in PBS containing 50% glycerol, and 0.02% sodium azide.

Source：Polyclonal, Rabbit,IgG

Dilution：WB 1:500-2000 ELISA 1:5000-20000

Purification：The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration：1 mg/ml

Storage Stability：-15°C to -25°C/1 year(Do not lower than -25°C)

Observed Band(KD)：60kD

Background： The protein encoded by this gene may interact with p53 and may be involved in tumorigenesis. The encoded protein also appears to be important for stem cell proliferation. This protein is found in both the nucleus and nucleolus. Three transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Nov 2010],

Function：domain:In contrast to other GTP-binding proteins, this family is characterized by a circular permutation of the GTPase motifs described by a G4-G1-G3 pattern.,domain:The basic domain (B) allows nucleolar localization in the absence of GTP. The intermediate domain (I) inhibits nucleolar localization by the B domain and is required for exit from the nucleolus. Exit from the nucleolus to the nucleoplasm requires both the I and the acidic (A) domains, and may be triggered by GTP hydrolysis.,function:May be required to maintain the proliferative capacity of stem cells and may play an important role in tumorigenesis.,similarity:Belongs to the MMR1/HSR1 GTP-binding protein family.,subcellular location:Shuttles between the nucleus and nucleolus.,subunit:May interact with p53/TP53 via its basic domain.,tissue specificity:Increased levels in lung tissue in cancer patients.,

Subcellular Location：Nucleus . Nucleus, nucleolus . Shuttles between the nucleus and nucleolus. .

Expression：Increased levels in lung tissue in cancer patients.