Target：RO52

Fields：Systemic lupus erythematosus

Gene Name：TRIM21 RNF81 RO52 SSA1

Protein Name：E3 ubiquitin-protein ligase TRIM21 (EC 6.3.2.-) (52 kDa Ro protein) (52 kDa ribonucleoprotein autoantigen Ro/SS-A) (RING finger protein 81) (Ro(SS-A)) (Sjoegren syndrome type A antigen) (SS-A) (Tripar

Human Gene Id：6737

Human Swiss Prot No：P19474

Mouse Swiss Prot No：Q62191

Immunogen：Synthesized peptide derived from human protein . at AA range: 140-220

Specificity：RO52 Polyclonal Antibody detects endogenous levels of protein.

Formulation：Liquid in PBS containing 50% glycerol, and 0.02% sodium azide.

Source：Polyclonal, Rabbit,IgG

Dilution：WB 1:500-2000 ELISA 1:5000-20000

Purification：The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration：1 mg/ml

Storage Stability：-15°C to -25°C/1 year(Do not lower than -25°C)

Observed Band(KD)：52kD

Background： This gene encodes a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. The encoded protein is part of the RoSSA ribonucleoprotein, which includes a single polypeptide and one of four small RNA molecules. The RoSSA particle localizes to both the cytoplasm and the nucleus. RoSSA interacts with autoantigens in patients with Sjogren syndrome and systemic lupus erythematosus. Alternatively spliced transcript variants for this gene have been described but the full-length nature of only one has been determined. [provided by RefSeq, Jul 2008],

Function：disease:Sera from patients with systemic lupus erythematosus (SLE) or primary Sjoegren syndrome often contain antibodies that react with normal cellular RO protein as if this antigen was foreign.,function:Ribonucleoprotein particle composed of a single polypeptide and one of four small RNA molecules. It is present in all mammalian cells studied but has no known function. At least two isoforms are present in nucleated and red blood cells, and tissue specific differences in RO/SSA proteins have been identified. The common feature of these proteins is their ability to bind HY RNAs.,similarity:Belongs to the TRIM/RBCC family.,similarity:Contains 1 B box-type zinc finger.,similarity:Contains 1 B30.2/SPRY domain.,similarity:Contains 1 RING-type zinc finger.,

Subcellular Location：Cytoplasm . Cytoplasmic vesicle, autophagosome . Nucleus . Cytoplasm, P-body . Enters the nucleus upon exposure to nitric oxide. Localizes to small dot- or rod-like structures in the cytoplasm, called processing bodies (P-bodies) that are located underneath the plasma membrane and also diffusely in the cytoplasm. They are located along the microtubules and are highly motile in cells. Colocalizes with DCP2 in P-bodies.

Expression：Isoform 1 and isoform 2 are expressed in fetal and adult heart and fetal lung.