Target:RNF5
Fields:Protein processing in endoplasmic reticulum
Gene Name:RNF5 G16 NG2 RMA1
Protein Name:E3 ubiquitin-protein ligase RNF5 (EC 6.3.2.-) (Protein G16) (RING finger protein 5) (Ram1 homolog) (HsRma1)
Human Gene Id:6048
Human Swiss Prot No:Q99942
Mouse Swiss Prot No:O35445
Rat Swiss Prot No:Q5M807
Immunogen:Synthesized peptide derived from part region of human protein
Specificity:RNF5 Polyclonal Antibody detects endogenous levels of protein.
Formulation:Liquid in PBS containing 50% glycerol, and 0.02% sodium azide.
Source:Polyclonal, Rabbit,IgG
Dilution:WB 1:500-2000 ELISA 1:5000-20000
Purification:The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration:1 mg/ml
Storage Stability:-15°C to -25°C/1 year(Do not lower than -25°C)
Observed Band(KD):19kD
Background: The protein encoded by this gene contains a RING finger, which is a motif known to be involved in protein-protein interactions. This protein is a membrane-bound ubiquitin ligase. It can regulate cell motility by targeting paxillin ubiquitination and altering the distribution and localization of paxillin in cytoplasm and cell focal adhesions. [provided by RefSeq, Jul 2008],
Function:function:Has E2-dependent E3 ubiquitin-protein ligase activity. May function together with E2 ubiquitin-conjugating enzymes UBE2D1/UBCH5A and UBE2D2/UBC4. Mediates ubiquitination of PXN/paxillin and Salmonella type III secreted protein sopA. May be involved in regulation of cell motility and localization of PXN/paxillin.,similarity:Contains 1 RING-type zinc finger.,subcellular location:Predominantly located in the plasma membrane, with some localization occurring within cytoplasmic organelles.,subunit:Interacts with PXN. Interacts with Salmonella typhimurium sopA. Interacts with JAMP.,tissue specificity:Widely expressed.,
Subcellular Location:Cell membrane ; Multi-pass membrane protein . Mitochondrion membrane ; Multi-pass membrane protein . Endoplasmic reticulum membrane ; Multi-pass membrane protein . Predominantly located in the plasma membrane, with some localization occurring within cytoplasmic organelles. .
Expression:Widely expressed.