Target：N/H/K-Ras

Fields：EGFR tyrosine kinase inhibitor resistance;Endocrine resistance;MAPK signaling pathway;ErbB signaling pathway;Ras signaling pathway;Rap1 signaling pathway;Chemokine signaling pathway;FoxO signaling pathway;Sphingolipid signaling pathway;Phospholipase D signaling pathway;Mitophagy - animal;Autophagy - animal;mTOR signaling pathway;PI3K-Akt signaling pathway;Apoptosis;Longevity regulating pathway;Longevity regulating pathway - multiple species;Cellular senescence;Axon guidance;VEGF signaling pathway;Apelin signaling pathway;Gap junction;Signaling pathways regulating pluripotency of stem cells;C-type lectin receptor signaling pathway;Natural killer cell mediated cytotoxicity;T cell receptor signaling pathway;B cell receptor signaling pathway;Fc epsilon RI signaling pathway;Thermogenesis;Long-term potentiation;Neurotrophin signaling pathway;Cholinergic synapse;Serotonergic synapse;Long-term depression;Regulation of actin

Gene Name：NRAS/HRAS/KRAS

Protein Name：GTPase Nras/GTPase Hras/GTPase Kras

Human Gene Id：3265/3845/4893

Human Swiss Prot No：P01111/P01112/P01116

Mouse Gene Id：15461/16653

Rat Gene Id：24605/293621/24525

Rat Swiss Prot No：Q04970/P20171/P08644

Immunogen：The antiserum was produced against synthesized peptide derived from human RASH/RASK. AA range:1-50

Specificity：N/H/K-Ras Polyclonal Antibody detects endogenous levels of N/H/K-Ras protein.

Formulation：Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Source：Polyclonal, Rabbit,IgG

Dilution：WB 1:500 - 1:2000. IHC: 1:100-300 ELISA: 1:20000. IF 1:100-300 Not yet tested in other applications.

Purification：The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration：1 mg/ml

Storage Stability：-15°C to -25°C/1 year(Do not lower than -25°C)

Other Name：NRAS;HRAS1;GTPase NRas;Transforming protein N-Ras;HRAS;HRAS1;GTPase HRas;H-Ras-1;Ha-Ras;Transforming protein p21;c-H-ras;p21ras;KRAS;KRAS2;RASK2;GTPase KRas;K-Ras 2;Ki-Ras;c-K-ras;c-Ki-ras

Observed Band(KD)：21kD

Background： This is an N-ras oncogene encoding a membrane protein that shuttles between the Golgi apparatus and the plasma membrane. This shuttling is regulated through palmitoylation and depalmitoylation by the ZDHHC9-GOLGA7 complex. The encoded protein, which has intrinsic GTPase activity, is activated by a guanine nucleotide-exchange factor and inactivated by a GTPase activating protein. Mutations in this gene have been associated with somatic rectal cancer, follicular thyroid cancer, autoimmune lymphoproliferative syndrome, Noonan syndrome, and juvenile myelomonocytic leukemia. [provided by RefSeq, Jun 2011],

Function：disease:Defects in NRAS are a cause of juvenile myelomonocytic leukemia (JMML) [MIM:607785]. JMML is a pediatric myelodysplastic syndrome that constitutes approximately 30% of childhood cases of myelodysplastic syndrome (MDS) and 2% of leukemia.,disease:Mutations which change AA 12, 13 or 61 activate the potential of Ras to transform cultured cells and are implicated in a variety of human tumors.,enzyme regulation:Alternate between an inactive form bound to GDP and an active form bound to GTP. Activated by a guanine nucleotide-exchange factor (GEF) and inactivated by a GTPase-activating protein (GAP).,function:Ras proteins bind GDP/GTP and possess intrinsic GTPase activity.,online information:NRAS mutation db,online information:RAS proteins entry,PTM:Palmitoylated by the ZDHHC9-GOLGA7 complex. A continuous cycle of de- and re-palmitoylation regulates rapid exchange between plasma membran

Subcellular Location：Cell membrane ; Lipid-anchor ; Cytoplasmic side . Golgi apparatus membrane ; Lipid-anchor . Shuttles between the plasma membrane and the Golgi apparatus. .

Expression： Bone marrow,Bone-marrow,Brain,Fibrosarcoma,Kidney,Leukemia,Lung car