Target：M-RIP

Gene Name：MPRIP

Protein Name：Myosin phosphatase Rho-interacting protein

Human Gene Id：23164/23164

Human Swiss Prot No：Q6WCQ1

Mouse Gene Id：26936

Mouse Swiss Prot No：P97434

Rat Gene Id：116504

Rat Swiss Prot No：Q9ERE6

Immunogen：Synthesized peptide derived from the Internal region of human M-RIP.

Specificity：M-RIP Polyclonal Antibody detects endogenous levels of M-RIP protein.

Formulation：Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Source：Polyclonal, Rabbit,IgG

Dilution：WB 1:500-2000;IHC 1:50-300; ELISA 2000-20000

Purification：The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration：1 mg/ml

Storage Stability：-15°C to -25°C/1 year(Do not lower than -25°C)

Other Name：MPRIP;KIAA0864;MRIP;RHOIP3;Myosin phosphatase Rho-interacting protein;M-RIP;Rho-interacting protein 3;RIP3;p116Rip

Observed Band(KD)：115kD

Background：function:Targets myosin phosphatase to the actin cytoskeleton. Required for the regulation of the actin cytoskeleton by RhoA and ROCK1. Depletion leads to an increased number of stress fibers in smooth muscle cells through stabilization of actin fibers by phosphorylated myosin. Overexpression of MRIP as well as its F-actin-binding region leads to disassembly of stress fibers in neuronal cells.,similarity:Contains 1 PH domain.,similarity:Contains 2 PH domains.,subcellular location:Colocalizes with F-actin.,subunit:Binds F-actin through its N-terminus (By similarity). Binds RHOA, PPP1R12A/MBS and PPP1R12C/MBS85 through adjacent coiled coil domains.,

Function：function:Targets myosin phosphatase to the actin cytoskeleton. Required for the regulation of the actin cytoskeleton by RhoA and ROCK1. Depletion leads to an increased number of stress fibers in smooth muscle cells through stabilization of actin fibers by phosphorylated myosin. Overexpression of MRIP as well as its F-actin-binding region leads to disassembly of stress fibers in neuronal cells.,similarity:Contains 1 PH domain.,similarity:Contains 2 PH domains.,subcellular location:Colocalizes with F-actin.,subunit:Binds F-actin through its N-terminus (By similarity). Binds RHOA, PPP1R12A/MBS and PPP1R12C/MBS85 through adjacent coiled coil domains.,

Subcellular Location：Cytoplasm, cytoskeleton . Colocalizes with F-actin.

Expression： Aorta,Brain,Epithelium,Lymph,Melanoma,Muscle,Ovary,Tongue,