Target：MRCKβ

Gene Name：CDC42BPB

Protein Name：Serine/threonine-protein kinase MRCK beta

Human Gene Id：9578

Human Swiss Prot No：Q9Y5S2

Mouse Gene Id：217866

Mouse Swiss Prot No：Q7TT50

Rat Gene Id：113960

Rat Swiss Prot No：Q7TT49

Immunogen：The antiserum was produced against synthesized peptide derived from human MRCKB. AA range:1641-1690

Specificity：MRCKβ Polyclonal Antibody detects endogenous levels of MRCKβ protein.

Formulation：Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Source：Polyclonal, Rabbit,IgG

Dilution：WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:40000.. IF 1:50-200

Purification：The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration：1 mg/ml

Storage Stability：-15°C to -25°C/1 year(Do not lower than -25°C)

Other Name：CDC42BPB;KIAA1124;Serine/threonine-protein kinase MRCK beta;CDC42-binding protein kinase beta;CDC42BP-beta;DMPK-like beta;Myotonic dystrophy kinase-related CDC42-binding kinase beta;MRCK beta;Myotonic dystrophy protein kinase-like b

Observed Band(KD)：194kD

Background： This gene encodes a member of the serine/threonine protein kinase family. The encoded protein contains a Cdc42/Rac-binding p21 binding domain resembling that of PAK kinase. The kinase domain of this protein is most closely related to that of myotonic dystrophy kinase-related ROK. Studies of the similar gene in rat suggested that this kinase may act as a downstream effector of Cdc42 in cytoskeletal reorganization. [provided by RefSeq, Jul 2008],

Function：catalytic activity:ATP + a protein = ADP + a phosphoprotein.,cofactor:Magnesium.,enzyme regulation:Maintained in an inactive, closed conformation by an interaction between the kinase domain and the negative autoregulatory C-terminal coiled-coil region. Agonist binding to the phorbol ester binding site disrupts this, releasing the kinase domain to allow N-terminus-mediated dimerization and kinase activation by transautophosphorylation.,function:May act as a downstream effector of CDC42 in cytoskeletal reorganization. Contributes to the actomyosin contractility required for cell invasion, through the regulation of MYPT1 and thus MLC2 phosphorylation.,sequence caution:Contaminating sequence. Potential poly-A sequence.,similarity:Belongs to the protein kinase superfamily.,similarity:Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. DMPK subfamily.,similarity:Conta

Subcellular Location：Cytoplasm . Cell membrane ; Peripheral membrane protein ; Cytoplasmic side . Cell junction . Cell projection, lamellipodium . Displays a dispersed punctate distribution and concentrates along the cell periphery, especially at the leading edge and cell-cell junction. This concentration is PH-domain dependent (By similarity). Detected at the leading edge of migrating cells. Localization at the leading edge of migrating cells requires interaction with catalytically active CDC42 (PubMed:21240187). Localizes in the lamellipodium in a FAM89B/LRAP25-dependent manner (By similarity). .

Expression：Expressed in all tissues examined, with high levels in heart, brain, placenta and lung.