Target：MRCKα

Gene Name：CDC42BPA

Protein Name：Serine/threonine-protein kinase MRCK alpha

Human Gene Id：8476

Human Swiss Prot No：Q5VT25

Mouse Swiss Prot No：Q3UU96

Rat Gene Id：114116

Rat Swiss Prot No：O54874

Immunogen：Synthesized peptide derived from the Internal region of human MRCKα. AA range: 580-660

Specificity：MRCKα Polyclonal Antibody detects endogenous levels of MRCKα protein.

Formulation：Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Source：Polyclonal, Rabbit,IgG

Dilution：WB 1:500-2000;IHC 1:50-300; ELISA 2000-20000

Purification：The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration：1 mg/ml

Storage Stability：-15°C to -25°C/1 year(Do not lower than -25°C)

Other Name：CDC42BPA;KIAA0451;Serine/threonine-protein kinase MRCK alpha;CDC42-binding protein kinase alpha;DMPK-like alpha;Myotonic dystrophy kinase-related CDC42-binding kinase alpha;MRCK alpha;Myotonic dystrophy protein kinase-like alpha

Observed Band(KD)：200kD

Background： The protein encoded by this gene is a member of the Serine/Threonine protein kinase family. This kinase contains multiple functional domains. Its kinase domain is highly similar to that of the myotonic dystrophy protein kinase (DMPK). This kinase also contains a Rac interactive binding (CRIB) domain, and has been shown to bind CDC42. It may function as a CDC42 downstream effector mediating CDC42 induced peripheral actin formation, and promoting cytoskeletal reorganization. Multiple alternatively spliced transcript variants have been described, and the full-length nature of two of them has been reported. [provided by RefSeq, Jul 2008],

Function：catalytic activity:ATP + a protein = ADP + a phosphoprotein.,cofactor:Magnesium.,enzyme regulation:Maintained in an inactive, closed conformation by an interaction between the kinase domain and the negative autoregulatory C-terminal coiled-coil region. Agonist binding to the phorbol ester binding site disrupts this, releasing the kinase domain to allow N-terminus-mediated dimerization and kinase activation by transautophosphorylation.,function:May act as a downstream effector of CDC42 in cytoskeletal reorganization. Contributes to the actomyosin contractility required for cell invasion, through the regulation of MYPT1 and thus MLC2 phosphorylation.,similarity:Belongs to the protein kinase superfamily.,similarity:Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. DMPK subfamily.,similarity:Contains 1 AGC-kinase C-terminal domain.,similarity:Contains 1 CNH domain

Subcellular Location：Cytoplasm . Cell projection, lamellipodium . Displays a dispersed punctate distribution and concentrates along the cell periphery, especially at the leading edge and cell-cell junction. This concentration is PH-domain dependent. Localizes in the lamellipodium in a FAM89B/LRAP25-dependent manner. .

Expression：Abundant in the heart, brain, skeletal muscle, kidney, and pancreas, with little or no expression in the lung and liver.