Target:Rho H
Fields:Leukocyte transendothelial migration;Salmonella infection
Gene Name:RhoH
Protein Name:Rho-related GTP-binding protein RhoH
Human Gene Id:399
Human Swiss Prot No:Q15669
Mouse Gene Id:74734
Mouse Swiss Prot No:Q9D3G9
Immunogen:The antiserum was produced against synthesized peptide derived from human RhoH. AA range:141-190
Specificity:Rho H Polyclonal Antibody detects endogenous levels of Rho H protein.
Formulation:Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Source:Polyclonal, Rabbit,IgG
Dilution:WB 1:500 - 1:2000. IHC 1:100 - 1:300. IF 1:200 - 1:1000. ELISA: 1:20000. Not yet tested in other applications.
Purification:The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration:1 mg/ml
Storage Stability:-15°C to -25°C/1 year(Do not lower than -25°C)
Other Name:RHOH;ARHH;TTF;Rho-related GTP-binding protein RhoH;GTP-binding protein TTF;Translocation three four protein
Observed Band(KD):21kD
Background: The protein encoded by this gene is a member of the Ras superfamily of guanosine triphosphate (GTP)-metabolizing enzymes. The encoded protein is expressed in hematopoietic cells, where it functions as a negative regulator of cell growth and survival. This gene may be hypermutated or misexpressed in leukemias and lymphomas. Chromosomal translocations in non-Hodgkin's lymphoma occur between this locus and B-cell CLL/lymphoma 6 (BCL6) on chromosome 3, leading to the production of fusion transcripts. Alternative splicing in the 5' untranslated region results in multiple transcript variants that encode the same protein. [provided by RefSeq, May 2013],
Function:disease:A chromosomal aberration involving RHOH is found in a non-Hodgkin lymphoma cell line. Translocation t(3;4)(q27;p11) with BCL6.,similarity:Belongs to the small GTPase superfamily. Rho family.,tissue specificity:Transcribed only in hemopoietic cells.,
Subcellular Location:Cytoplasm . Cell membrane ; Lipid-anchor ; Cytoplasmic side . Colocalizes together with ZAP70 in the immunological synapse. .
Expression:Expressed only in hematopoietic cells. Present at very high levels in the thymus, less abundant in the spleen, and least abundant in the bone marrow. Expressed at a higher level in the TH1 subtype of T-helper cells than in the TH2 subpopulation. Expressed in neutrophils under inflammatory conditions, such as cystic fibrosis, ulcerative colitis and appendicitis.