Target：PDE5A

Fields：Purine metabolism;Metabolic pathways;cGMP-PKG signaling pathway

Gene Name：PDE5A PDE5

Protein Name：cGMP-specific 3',5'-cyclic phosphodiesterase (EC 3.1.4.35) (cGMP-binding cGMP-specific phosphodiesterase) (CGB-PDE)

Human Gene Id：8654

Human Swiss Prot No：O76074

Mouse Swiss Prot No：Q8CG03

Rat Swiss Prot No：O54735

Immunogen：Synthesized peptide derived from part region of human protein

Specificity：PDE5A Polyclonal Antibody detects endogenous levels of protein.

Formulation：Liquid in PBS containing 50% glycerol, and 0.02% sodium azide.

Source：Polyclonal, Rabbit,IgG

Dilution：WB 1:500-2000 ELISA 1:5000-20000

Purification：The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration：1 mg/ml

Storage Stability：-15°C to -25°C/1 year(Do not lower than -25°C)

Observed Band(KD)：96kD

Background： This gene encodes a cGMP-binding, cGMP-specific phosphodiesterase, a member of the cyclic nucleotide phosphodiesterase family. This phosphodiesterase specifically hydrolyzes cGMP to 5'-GMP. It is involved in the regulation of intracellular concentrations of cyclic nucleotides and is important for smooth muscle relaxation in the cardiovascular system. Alternative splicing of this gene results in three transcript variants encoding distinct isoforms. [provided by RefSeq, Jul 2008],

Function：catalytic activity:Guanosine 3',5'-cyclic phosphate + H(2)O = guanosine 5'-phosphate.,cofactor:Divalent cations. Zinc ions yields maximum activity. Manganese, magnesium and cobalt also support catalysis but at much higher concentrations.,domain:Composed of a C-terminal catalytic domain containing two putative divalent metal sites and an N-terminal regulatory domain which contains two homologous allosteric cGMP-binding regions, A and B.,enzyme regulation:Sildenafil (Viagra) is a highly selective and potent inhibitor of PDE5A and is effective in the treatment of penile erectile dysfunction. Also inhibited by zaprinast.,function:Plays a role in signal transduction by regulating the intracellular concentration of cyclic nucleotides. This phosphodiesterase catalyzes the specific hydrolysis of cGMP to 5'-GMP.,pathway:Purine metabolism; cGMP degradation; GMP from cGMP: step 1/1.,PTM:Phosphoryla

Subcellular Location：cytosol,

Expression：Expressed in aortic smooth muscle cells, heart, placenta, skeletal muscle and pancreas and, to a much lesser extent, in brain, liver and lung.