Target：PC-PLD3

Fields：Glycerophospholipid metabolism;Ether lipid metabolism;Metabolic pathways

Gene Name：PLD3

Protein Name：Phospholipase D3

Human Gene Id：23646

Human Swiss Prot No：Q8IV08

Mouse Gene Id：18807

Mouse Swiss Prot No：O35405

Rat Gene Id：361527

Rat Swiss Prot No：Q5FVH2

Immunogen：The antiserum was produced against synthesized peptide derived from human PLD3. AA range:326-375

Specificity：PC-PLD3 Polyclonal Antibody detects endogenous levels of PC-PLD3 protein.

Formulation：Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Source：Polyclonal, Rabbit,IgG

Dilution：IHC 1:100 - 1:300. ELISA: 1:5000.. IF 1:50-200

Purification：The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration：1 mg/ml

Storage Stability：-15°C to -25°C/1 year(Do not lower than -25°C)

Other Name：PLD3;Phospholipase D3;PLD 3;Choline phosphatase 3;HindIII K4L homolog;Hu-K4;Phosphatidylcholine-hydrolyzing phospholipase D3

Molecular Weight(Da)：55kD

Background： This gene encodes a member of the phospholipase D (PLD) family of enzymes that catalyze the hydrolysis of membrane phospholipids. The encoded protein is a single-pass type II membrane protein and contains two PLD phosphodiesterase domains. This protein influences processing of amyloid-beta precursor protein. Mutations in this gene are associated with Alzheimer disease risk. Alternatively spliced transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Apr 2014],

Function：catalytic activity:A phosphatidylcholine + H(2)O = choline + a phosphatidate.,PTM:Glycosylated.,similarity:Belongs to the phospholipase D family.,similarity:Contains 2 PLD phosphodiesterase domains.,tissue specificity:Widely expressed. Expressec at higher level in brain. Expresse at low level in corpus callosum, suggsting that it is highly expressed in neurons.,

Subcellular Location：Endoplasmic reticulum membrane ; Single-pass type II membrane protein . Lysosome lumen . Early endosome membrane ; Single-pass type II membrane protein . Late endosome membrane ; Single-pass type II membrane protein . Golgi apparatus membrane ; Single-pass type II membrane protein . Endosome membrane ; Single-pass type II membrane protein . Localizes to ER-associated vesicles in differentiating myotubes (PubMed:22428023). The soluble form in lysosome arises by proteolytic processing of the membrane-bound form (PubMed:29386126). Colocalizes with APP in endosomes (PubMed:29368044). .

Expression：Widely expressed. In the brain, high levels of expression are detected in the frontal, temporal and occipital cortices and hippocampus. Expressed at low level in corpus callosum.