Target：PIXβ

Fields：Regulation of actin cytoskeleton;Yersinia infection

Gene Name：ARHGEF7

Protein Name：Rho guanine nucleotide exchange factor 7

Human Gene Id：8874

Human Swiss Prot No：Q14155

Mouse Gene Id：54126

Mouse Swiss Prot No：Q9ES28

Rat Gene Id：114559

Rat Swiss Prot No：O55043

Immunogen：The antiserum was produced against synthesized peptide derived from human ARHGEF7. AA range:311-360

Specificity：PIXβ Polyclonal Antibody detects endogenous levels of PIXβ protein.

Formulation：Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Source：Polyclonal, Rabbit,IgG

Dilution：WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:5000.. IF 1:50-200

Purification：The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration：1 mg/ml

Storage Stability：-15°C to -25°C/1 year(Do not lower than -25°C)

Other Name：ARHGEF7;COOL1;KIAA0142;P85SPR;PAK3BP;PIXB;Nbla10314;Rho guanine nucleotide exchange factor 7;Beta-Pix;COOL-1;PAK-interacting exchange factor beta;p85

Observed Band(KD)：80kD

Background： This gene encodes a protein that belongs to a family of cytoplasmic proteins that activate the Ras-like family of Rho proteins by exchanging bound GDP for GTP. It forms a complex with the small GTP binding protein Rac1 and recruits Rac1 to membrane ruffles and to focal adhesions. Multiple alternatively spliced transcript variants encoding different isoforms have been observed for this gene. [provided by RefSeq, Mar 2016],

Function：function:Acts as a RAC1 guanine nucleotide exchange factor (GEF) and can induce membrane ruffling.,similarity:Contains 1 CH (calponin-homology) domain.,similarity:Contains 1 DH (DBL-homology) domain.,similarity:Contains 1 PH domain.,similarity:Contains 1 SH3 domain.,subunit:Interacts with PAK kinases through the SH3 domain. Interacts with GIT1 and TGFB1I1. Interacts with ITCH (By similarity). Interacts with unphosphorylated PAK1.,

Subcellular Location：Cell junction, focal adhesion . Cell projection, ruffle . Cytoplasm, cell cortex . Cell projection, lamellipodium . Detected at cell adhesions. A small proportion is detected at focal adhesions.

Expression： Amygdala,Bone marrow,Brain,Epithelium,Neuroblastoma,Testis,