Target：PIAS1

Fields：Ubiquitin mediated proteolysis;JAK-STAT signaling pathway;Hepatitis C

Gene Name：PIAS1

Protein Name：E3 SUMO-protein ligase PIAS1

Human Gene Id：8554

Human Swiss Prot No：O75925

Mouse Gene Id：56469

Mouse Swiss Prot No：O88907

Immunogen：The antiserum was produced against synthesized peptide derived from human PIAS1. AA range:10-59

Specificity：PIAS 1 Polyclonal Antibody detects endogenous levels of PIAS 1 protein.

Formulation：Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Source：Polyclonal, Rabbit,IgG

Dilution：WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:10000.. IF 1:50-200

Purification：The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration：1 mg/ml

Storage Stability：-15°C to -25°C/1 year(Do not lower than -25°C)

Other Name：PIAS1;DDXBP1;E3 SUMO-protein ligase PIAS1;DEAD/H box-binding protein 1;Gu-binding protein;GBP;Protein inhibitor of activated STAT protein 1;RNA helicase II-binding protein

Observed Band(KD)：72kD

Background： This gene encodes a member of the protein inhibitor of activated STAT (PIAS) family. PIAS proteins function as SUMO E3 ligases and play important roles in many cellular processes by mediating the sumoylation of target proteins. This protein plays a central role as a transcriptional coregulator of numerous cellular pathways includign the STAT1 and nuclear factor kappaB pathways. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Mar 2016],

Function：domain:The LXXLL motif is a transcriptional coregulator signature.,domain:The SP-RING-type domain is required for promoting EKLF sumoylation.,function:Functions as an E3-type small ubiquitin-like modifier (SUMO) ligase, stabilizing the interaction between UBE2I and the substrate, and as a SUMO-tethering factor. Plays a crucial role as a transcriptional coregulation in various cellular pathways, including the STAT pathway, the p53 pathway and the steroid hormone signaling pathway. The effects of this transcriptional coregulation, transactivation or silencing, may vary depending upon the biological context.,pathway:Protein modification; protein sumoylation.,PTM:Sumoylated.,similarity:Belongs to the PIAS family.,similarity:Contains 1 SAP domain.,similarity:Contains 1 SP-RING-type zinc finger.,subcellular location:Interaction with CSRP2 may induce a partial redistribution along the cytoskele

Subcellular Location：Nucleus speckle . Nucleus, PML body . Interaction with CSRP2 may induce a partial redistribution along the cytoskeleton.

Expression：Expressed in numerous tissues with highest level in testis.