Target：Rab 5A

Fields：Ras signaling pathway;Endocytosis;Phagosome;Vasopressin-regulated water reabsorption;Amyotrophic lateral sclerosis;Pathways of neurodegeneration - multiple diseases;Salmonella infection;Amoebiasis;Tuberculosis

Gene Name：RAB5A

Protein Name：Ras-related protein Rab-5A

Human Gene Id：5868

Human Swiss Prot No：P20339

Mouse Gene Id：271457

Mouse Swiss Prot No：Q9CQD1

Immunogen：The antiserum was produced against synthesized peptide derived from the Internal region of human RAB5A. AA range:71-120

Specificity：Rab 5A Polyclonal Antibody detects endogenous levels of Rab 5A protein.

Formulation：Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Source：Polyclonal, Rabbit,IgG

Dilution：WB 1:500 - 1:2000. IHC: 1:100-1:300. ELISA: 1:20000.. IF 1:50-200

Purification：The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration：1 mg/ml

Storage Stability：-15°C to -25°C/1 year(Do not lower than -25°C)

Other Name：RAB5A;RAB5;Ras-related protein Rab-5A

Observed Band(KD)：24kD

Background：enzyme regulation:Regulated by guanine nucleotide exchange factors (GEFs) which promote the exchange of bound GDP for free GTP.,function:Required for the fusion of plasma membranes and early endosomes.,similarity:Belongs to the small GTPase superfamily. Rab family.,subcellular location:Enriched in stage I melanosomes.,subunit:Binds EEA1. Interacts with RIN1 and GAPVD1, which regulate its pathway, probably by acting as a GEF. Interacts with ALS2CL, UNC84B, ZFYVE20 and RUFY1. Interacts with SGSM1 and SGSM3.,

Function：enzyme regulation:Regulated by guanine nucleotide exchange factors (GEFs) which promote the exchange of bound GDP for free GTP.,function:Required for the fusion of plasma membranes and early endosomes.,similarity:Belongs to the small GTPase superfamily. Rab family.,subcellular location:Enriched in stage I melanosomes.,subunit:Binds EEA1. Interacts with RIN1 and GAPVD1, which regulate its pathway, probably by acting as a GEF. Interacts with ALS2CL, UNC84B, ZFYVE20 and RUFY1. Interacts with SGSM1 and SGSM3.,

Subcellular Location：Cell membrane ; Lipid-anchor ; Cytoplasmic side . Early endosome membrane ; Lipid-anchor . Melanosome . Cytoplasmic vesicle . Cell projection, ruffle . Membrane . Cytoplasm, cytosol . Cytoplasmic vesicle, phagosome membrane . Endosome membrane . Enriched in stage I melanosomes (PubMed:17081065). Alternates between membrane-bound and cytosolic forms (Probable). .

Expression： Brain,Cervix,Cervix carcinoma,Placenta,