Target:Rab 35
Fields:Endocytosis
Gene Name:RAB35
Protein Name:Ras-related protein Rab-35
Human Gene Id:11021
Human Swiss Prot No:Q15286
Mouse Gene Id:77407
Mouse Swiss Prot No:Q6PHN9
Rat Gene Id:288700
Rat Swiss Prot No:Q5U316
Immunogen:The antiserum was produced against synthesized peptide derived from human RAB35. AA range:99-148
Specificity:Rab 35 Polyclonal Antibody detects endogenous levels of Rab 35 protein.
Formulation:Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Source:Polyclonal, Rabbit,IgG
Dilution:WB 1:500-2000;IHC 1:50-300; ELISA 2000-20000
Purification:The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration:1 mg/ml
Storage Stability:-15°C to -25°C/1 year(Do not lower than -25°C)
Other Name:RAB35;RAB1C;RAY;Ras-related protein Rab-35;GTP-binding protein RAY;Ras-related protein Rab-1C
Observed Band(KD):24kD
Background:function:Possesses GTPase activity.,similarity:Belongs to the small GTPase superfamily. Rab family.,subcellular location:Identified by mass spectrometry in melanosome fractions from stage I to stage IV.,
Function:function:Possesses GTPase activity.,similarity:Belongs to the small GTPase superfamily. Rab family.,subcellular location:Identified by mass spectrometry in melanosome fractions from stage I to stage IV.,
Subcellular Location:Cell membrane ; Lipid-anchor ; Cytoplasmic side . Membrane, clathrin-coated pit . Cytoplasmic vesicle, clathrin-coated vesicle . Endosome . Melanosome . Present on sorting endosomes and recycling endosome tubules (PubMed:16950109). Tends to be enriched in PIP2-positive cell membrane domains (PubMed:16950109). During mitosis, associated with the plasma membrane and present at the ingressing furrow during early cytokinesis as well as at the intercellular bridge later during cytokinesis (PubMed:16950109). Identified in stage I to stage IV melanosomes (PubMed:17081065). .
Expression: Brain,Muscle,Uterus,