Target：TDG

Fields：Base excision repair

Gene Name：TDG

Protein Name：G/T mismatch-specific thymine DNA glycosylase

Human Gene Id：6996

Human Swiss Prot No：Q13569

Mouse Swiss Prot No：P56581

Immunogen：The antiserum was produced against synthesized peptide derived from human TDG. AA range:31-80

Specificity：TDG Polyclonal Antibody detects endogenous levels of TDG protein.

Formulation：Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Source：Polyclonal, Rabbit,IgG

Dilution：WB 1:500 - 1:2000. IHC 1:100 - 1:300. IF 1:200 - 1:1000. ELISA: 1:5000. Not yet tested in other applications.

Purification：The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration：1 mg/ml

Storage Stability：-15°C to -25°C/1 year(Do not lower than -25°C)

Other Name：TDG;G/T mismatch-specific thymine DNA glycosylase;Thymine-DNA glycosylase

Observed Band(KD)：46kD

Background： The protein encoded by this gene belongs to the TDG/mug DNA glycosylase family. Thymine-DNA glycosylase (TDG) removes thymine moieties from G/T mismatches by hydrolyzing the carbon-nitrogen bond between the sugar-phosphate backbone of DNA and the mispaired thymine. With lower activity, this enzyme also removes thymine from C/T and T/T mispairings. TDG can also remove uracil and 5-bromouracil from mispairings with guanine. This enzyme plays a central role in cellular defense against genetic mutation caused by the spontaneous deamination of 5-methylcytosine and cytosine. This gene may have a pseudogene in the p arm of chromosome 12. [provided by RefSeq, Jul 2008],

Function：function:In the DNA of higher eukaryotes, hydrolytic deamination of 5-methylcytosine to thymine leads to the formation of G/T mismatches. This enzyme corrects G/T mispairs to G/C pairs. It is capable of hydrolyzing the carbon-nitrogen bond between the sugar-phosphate backbone of the DNA and a mispaired thymine. In addition to the G/T, it can remove thymine also from C/T and T/T mispairs in the order G/T C/T > T/T. It has no detectable activity on apyrimidinic sites and does not catalyze the removal of thymine from A/T pairs or from single-stranded DNA. It can also remove uracil and 5-bromouracil from mispairs with guanine.,PTM:Sumoylation on Lys-330 by either SUMO1 or SUMO2 induces dissociation of the product DNA.,similarity:Belongs to the TDG/mug DNA glycosylase family.,

Subcellular Location：Nucleus .

Expression： Colon,Lung,Pooled,Thymus,