Target：Rev-erbα

Fields：Circadian rhythm

Gene Name：NR1D1

Protein Name：Nuclear receptor subfamily 1 group D member 1

Human Gene Id：9572

Human Swiss Prot No：P20393

Mouse Swiss Prot No：Q3UV55

Immunogen：Synthesized peptide derived from Rev-erbα . at AA range: 350-430

Specificity：Rev-erbα Polyclonal Antibody detects endogenous levels of Rev-erbα protein.

Formulation：Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Source：Polyclonal, Rabbit,IgG

Dilution：WB 1:500-2000;IHC 1:50-300

Purification：The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration：1 mg/ml

Storage Stability：-15°C to -25°C/1 year(Do not lower than -25°C)

Other Name：NR1D1;EAR1;HREV;THRAL;Nuclear receptor subfamily 1 group D member 1;Rev-erbA-alpha;V-erbA-related protein 1;EAR-1

Observed Band(KD)：67kD

Background： This gene encodes a transcription factor that is a member of the nuclear receptor subfamily 1. The encoded protein is a ligand-sensitive transcription factor that negatively regulates the expression of core clock proteins. In particular this protein represses the circadian clock transcription factor aryl hydrocarbon receptor nuclear translocator-like protein 1 (ARNTL). This protein may also be involved in regulating genes that function in metabolic, inflammatory and cardiovascular processes. [provided by RefSeq, Jan 2013],

Function：domain:Composed of three domains: a modulating N-terminal domain, a DNA-binding domain and a C-terminal steroid-binding domain.,function:Functions as a constitutive transcriptional repressor. Possible receptor for triiodothyronine.,similarity:Belongs to the nuclear hormone receptor family. NR1 subfamily.,similarity:Contains 1 nuclear receptor DNA-binding domain.,subunit:Interacts with C1D.,tissue specificity:Expressed in all tissues and cell lines examined. Expressed at high levels in some squamous carcinoma cell lines.,

Subcellular Location：Nucleus . Cytoplasm . Cell projection, dendrite . Cell projection, dendritic spine . Localizes to the cytoplasm, dendrites and dendritic spine in the presence of OPHN1. Localizes predominantly to the nucleus at ZT8 whereas it is cytoplasmic at ZT20. Phosphorylation by CSNK1E enhances its cytoplasmic localization. .

Expression：Widely expressed. Expressed at high levels in the liver, adipose tissue, skeletal muscle and brain. Also expressed in endothelial cells (ECs), vascular smooth muscle cells (VSMCs) and macrophages. Expression oscillates diurnally in the suprachiasmatic nucleus (SCN) of the hypothalamus as well as in peripheral tissues. Expression increases during the differentiation of pre-adipocytes into mature adipocytes. Expressed at high levels in some squamous carcinoma cell lines.