Target：3β-HSD7

Fields：Primary bile acid biosynthesis;Metabolic pathways

Gene Name：HSD3B7

Protein Name：3 beta-hydroxysteroid dehydrogenase type 7

Human Gene Id：80270

Human Swiss Prot No：Q9H2F3

Mouse Gene Id：101502

Mouse Swiss Prot No：Q9EQC1

Rat Gene Id：246211

Rat Swiss Prot No：O35048

Immunogen：The antiserum was produced against synthesized peptide derived from human HSD3B7. AA range:121-170

Specificity：3β-HSD7 Polyclonal Antibody detects endogenous levels of 3β-HSD7 protein.

Formulation：Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Source：Polyclonal, Rabbit,IgG

Dilution：WB 1:500 - 1:2000. ELISA: 1:20000. Not yet tested in other applications.

Purification：The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration：1 mg/ml

Storage Stability：-15°C to -25°C/1 year(Do not lower than -25°C)

Other Name：HSD3B7;3 beta-hydroxysteroid dehydrogenase type 7;3 beta-hydroxysteroid dehydrogenase type VII;3-beta-HSD VII;3-beta-hydroxy-Delta(5)-C27 steroid oxidoreductase;C(27) 3-beta-HSD;Cholest-5-ene-3-beta;7-alpha-diol 3-beta-dehydrogenase

Observed Band(KD)：41kD

Background： This gene encodes an enzyme which is involved in the initial stages of the synthesis of bile acids from cholesterol and a member of the short-chain dehydrogenase/reductase superfamily. The encoded protein is a membrane-associated endoplasmic reticulum protein which is active against 7-alpha hydrosylated sterol substrates. Mutations in this gene are associated with a congenital bile acid synthesis defect which leads to neonatal cholestasis, a form of progressive liver disease. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Dec 2008],

Function：catalytic activity:3-beta-hydroxy-Delta(5)-steroid + NAD(+) = 3-oxo-Delta(5)-steroid + NADH.,catalytic activity:Cholest-5-ene-3-beta,7-alpha-diol + NAD(+) = 7-alpha-hydroxycholest-4-en-3-one + NADH.,disease:Defects in HSD3B7 are the cause of congenital bile acid synthesis defect type 1 (CBAS1) [MIM:607765]; also known as neonatal progressive intrahepatic cholestasis. CBAS1 is due to a primary defect in bile synthesis leading to progressive liver disease. Clinical features include neonatal jaundice, severe intrahepatic cholestasis and cirrhosis.,function:Plays a central role during spermatogenesis by repressing transposable elements and prevent their mobilization, which is essential for the germline integrity. Plays an essential role in meiotic differentiation of spermatocytes, germ cell differentiation and in self-renewal of spermatogonial stem cells. Its presence in oocytes suggests tha

Subcellular Location：Endoplasmic reticulum membrane; Multi-pass membrane protein.

Expression： Stomach,Testis,Uterus,