Target：IDH3A

Fields：Citrate cycle (TCA cycle);Metabolic pathways;Carbon metabolism;2-Oxocarboxylic acid metabolism;Biosynthesis of amino acids

Gene Name：IDH3A

Protein Name：Isocitrate dehydrogenase [NAD] subunit alpha mitochondrial

Human Gene Id：3419

Human Swiss Prot No：P50213

Mouse Gene Id：67834

Mouse Swiss Prot No：Q9D6R2

Rat Gene Id：114096

Rat Swiss Prot No：Q99NA5

Immunogen：Synthesized peptide derived from the Internal region of human IDH3A.

Specificity：IDH3A Polyclonal Antibody detects endogenous levels of IDH3A protein.

Formulation：Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Source：Polyclonal, Rabbit,IgG

Dilution：WB 1:500 - 1:2000. IHC: 1:100-1:300. ELISA: 1:20000.. IF 1:50-200

Purification：The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration：1 mg/ml

Storage Stability：-15°C to -25°C/1 year(Do not lower than -25°C)

Other Name：IDH3A;Isocitrate dehydrogenase [NAD] subunit alpha, mitochondrial;Isocitric dehydrogenase subunit alpha;NAD(+)-specific ICDH subunit alpha

Observed Band(KD)：39kD

Background： Isocitrate dehydrogenases catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. These enzymes belong to two distinct subclasses, one of which utilizes NAD(+) as the electron acceptor and the other NADP(+). Five isocitrate dehydrogenases have been reported: three NAD(+)-dependent isocitrate dehydrogenases, which localize to the mitochondrial matrix, and two NADP(+)-dependent isocitrate dehydrogenases, one of which is mitochondrial and the other predominantly cytosolic. NAD(+)-dependent isocitrate dehydrogenases catalyze the allosterically regulated rate-limiting step of the tricarboxylic acid cycle. Each isozyme is a heterotetramer that is composed of two alpha subunits, one beta subunit, and one gamma subunit. The protein encoded by this gene is the alpha subunit of one isozyme of NAD(+)-dependent isocitrate dehydrogenase. [provided by RefSeq, Jul 2008],

Function：catalytic activity:Isocitrate + NAD(+) = 2-oxoglutarate + CO(2) + NADH.,cofactor:Binds 1 magnesium or manganese ion per subunit.,similarity:Belongs to the isocitrate and isopropylmalate dehydrogenases family.,subunit:Heterooligomer of subunits alpha, beta, and gamma in the apparent ratio of 2:1:1.,

Subcellular Location：Mitochondrion.

Expression： Brain,Brain cortex,Cajal-Retzius cell,Esophagus tumor,Fetal