Target：IDH1

Fields：Citrate cycle (TCA cycle);Glutathione metabolism;Metabolic pathways;Carbon metabolism;2-Oxocarboxylic acid metabolism;Biosynthesis of amino acids;Peroxisome;Central carbon metabolism in cancer

Gene Name：IDH1

Protein Name：Isocitrate dehydrogenase [NADP] cytoplasmic

Human Gene Id：3417

Human Swiss Prot No：O75874

Mouse Gene Id：15926

Mouse Swiss Prot No：O88844

Rat Gene Id：24479

Rat Swiss Prot No：P41562

Immunogen：Synthesized peptide derived from the N-terminal region of human IDH1.

Specificity：IDH1 Polyclonal Antibody detects endogenous levels of IDH1 protein.

Formulation：Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Source：Polyclonal, Rabbit,IgG

Dilution：WB 1:500 - 1:2000. IHC: 1:100-1:300. ELISA: 1:20000.. IF 1:50-200

Purification：The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration：1 mg/ml

Storage Stability：-15°C to -25°C/1 year(Do not lower than -25°C)

Other Name：IDH1;PICD;Isocitrate dehydrogenase [NADP] cytoplasmic;IDH;Cytosolic NADP-isocitrate dehydrogenase;IDP;NADP(+)-specific ICDH;Oxalosuccinate decarboxylase

Observed Band(KD)：46kD

Background： Isocitrate dehydrogenases catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. These enzymes belong to two distinct subclasses, one of which utilizes NAD(+) as the electron acceptor and the other NADP(+). Five isocitrate dehydrogenases have been reported: three NAD(+)-dependent isocitrate dehydrogenases, which localize to the mitochondrial matrix, and two NADP(+)-dependent isocitrate dehydrogenases, one of which is mitochondrial and the other predominantly cytosolic. Each NADP(+)-dependent isozyme is a homodimer. The protein encoded by this gene is the NADP(+)-dependent isocitrate dehydrogenase found in the cytoplasm and peroxisomes. It contains the PTS-1 peroxisomal targeting signal sequence. The presence of this enzyme in peroxisomes suggests roles in the regeneration of NADPH for intraperoxisomal reductions, such as the conversion of 2, 4-dienoyl-CoAs to

Function：catalytic activity:Isocitrate + NADP(+) = 2-oxoglutarate + CO(2) + NADPH.,catalytic activity:Oxalosuccinate + NADP(+) = 2-oxoglutarate + CO(2) + NADPH.,cofactor:Binds 1 magnesium or manganese ion per subunit.,disease:Defects in IDH1 are a cause of glioblastoma multiforme (GBM) [MIM:137800]; also called familial glioma of brain. Gliomas are central nervous system neoplasms derived from glial cells and comprise astrocytomas, glioblastoma multiforme, oligodendrogliomas, and ependymomas.,miscellaneous:Cancer mutations affecting Arg-132 are tissue-specific, and suggest that this residue plays a unique role in the development of high-grade gliomas.,online information:Isocitrate dehydrogenase entry,similarity:Belongs to the isocitrate and isopropylmalate dehydrogenases family.,subunit:Homodimer.,

Subcellular Location：Cytoplasm, cytosol . Peroxisome .

Expression： Brain,Cajal-Retzius cell,Fetal brain cortex,Human endometri