Main Information

Target

HIPK1/2/3

Host Species

Rabbit

Reactivity

Human, Mouse, Rat

Applications

WB

MW

133kD (Calculated)

Conjugate/Modification

Phospho

Detailed Information

Recommended Dilution Ratio

WB 1:500-2000

Formulation

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Specificity

This antibody detects endogenous levels of HIPK1/2/3 (Phospho-Tyr352/361/359) at Human, Mouse,Rat

Purification

The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.

Storage

-15°C to -25°C/1 year(Do not lower than -25°C)

Concentration

1 mg/ml

MW(Calculated)

133kD

Modification

Phospho

Clonality

Polyclonal

Isotype

IgG

Antigen&Target Information

Immunogen:

Synthesized peptide derived from human HIPK1/2/3 (Phospho-Tyr352/361/359)

Specificity:

This antibody detects endogenous levels of HIPK1/2/3 (Phospho-Tyr352/361/359) at Human, Mouse,Rat

Gene Name:

HIPK1 KIAA0630 MYAK NBAK2

Protein Name:

HIPK1/2/3 (Phospho-Tyr352/361/359)

Other Name:

Homeodomain-interacting protein kinase 1 ;
Nuclear body-associated kinase 2 ;

Database Link:

Organism	Gene ID	SwissProt
Human	204851;	Q86Z02;
Mouse	15257;	O88904;
Rat		A4L9P5;

Background:

homeodomain interacting protein kinase 1(HIPK1) Homo sapiens The protein encoded by this gene belongs to the Ser/Thr family of protein kinases and HIPK subfamily. It phosphorylates homeodomain transcription factors and may also function as a co-repressor for homeodomain transcription factors. Alternative splicing results in four transcript variants encoding four distinct isoforms. [provided by RefSeq, Jul 2008],

Function:

Catalytic activity:ATP + a protein = ADP + a phosphoprotein.,Function:May play a role as a corepressor for homeodomain transcription factors. Phosphorylates DAXX in response to stress, and mediates its translocation from the nucleus to the cytoplasm. May be involved in malignant squamous cell tumor formation.,PTM:Autophosphorylated. Phosphorylated and activated by JNK1.,similarity:Belongs to the protein kinase superfamily.,similarity:Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. HIPK subfamily.,similarity:Contains 1 protein kinase domain.,subcellular location:Predominantly nuclear.,subunit:Interacts with Nkx1-2 and Nkx2-5 (By similarity). Interacts with DAXX and TP53.,tissue specificity:Ubiquitously expressed with highest levels in skeletal muscle and heart. Overexpressed in breast cancer cell lines.,

Cellular Localization:

Nucleus . Cytoplasm . Nucleus speckle . Predominantly nuclear. Translocates from nucleus to cytoplasm in response to stress stimuli via SENP1-mediated desumoylation. .

Tissue Expression:

Ubiquitously expressed with highest levels in skeletal muscle and heart. Overexpressed in breast cancer cell lines. Isoform 2 is highly expressed in testis. Expressed in both androgen-dependent and androgen-independent prostate cancer cells (PubMed:28289210).

Research Areas:

>>Cellular senescence