Main Information

Target

SPC24

Host Species

Rabbit

Reactivity

Human, Mouse

Applications

WB

MW

22kD (Calculated)

Conjugate/Modification

Unmodified

Detailed Information

Recommended Dilution Ratio

WB 1:500-2000

Formulation

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Specificity

This antibody detects endogenous levels of SPC24 at Human, Mouse

Purification

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Storage

-15°C to -25°C/1 year(Do not lower than -25°C)

Concentration

1 mg/ml

MW(Calculated)

22kD

Modification

Unmodified

Clonality

Polyclonal

Isotype

IgG

Antigen&Target Information

Immunogen:

Synthesized peptide derived from human SPC24

Specificity:

This antibody detects endogenous levels of SPC24 at Human, Mouse

Gene Name:

SPC24 SPBC24

Protein Name:

Kinetochore protein Spc24 (hSpc24)

Database Link:

Organism	Gene ID	SwissProt
Human	147841;	Q8NBT2;
Mouse	67629;	Q9D083;

Function:

Acts as a component of the essential kinetochore-associated NDC80 complex, which is required for chromosome segregation and spindle checkpoint activity . Required for kinetochore integrity and the organization of stable microtubule binding sites in the outer plate of the kinetochore . The NDC80 complex synergistically enhances the affinity of the SKA1 complex for microtubules and may allow the NDC80 complex to track depolymerizing microtubules .

Cellular Localization:

Nucleus . Chromosome, centromere, kinetochore . Localizes to kinetochores from late prophase to anaphase. Localizes specifically to the outer plate of the kinetochore. .