Main Information

Target

IST1

Host Species

Rabbit

Reactivity

Human, Mouse, Rat

Applications

WB

MW

40kD (Calculated)

Conjugate/Modification

Unmodified

Detailed Information

Recommended Dilution Ratio

WB 1:500-2000

Formulation

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Specificity

This antibody detects endogenous levels of hIST1 at Human, Mouse,Rat

Purification

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Storage

-15°C to -25°C/1 year(Do not lower than -25°C)

Concentration

1 mg/ml

MW(Calculated)

40kD

Modification

Unmodified

Clonality

Polyclonal

Isotype

IgG

Antigen&Target Information

Immunogen:

Synthesized peptide derived from human hIST1

Specificity:

This antibody detects endogenous levels of hIST1 at Human, Mouse,Rat

Gene Name:

IST1 KIAA0174

Protein Name:

IST1 homolog (hIST1) (Putative MAPK-activating protein PM28)

Database Link:

Organism	Gene ID	SwissProt
Human	9798;	P53990;
Mouse	71955;	Q9CX00;
Rat	307833;	Q568Z6;

Function:

ESCRT-III-like protein involved in cytokinesis, nuclear envelope reassembly and endosomal tubulation . Is required for efficient abscission during cytokinesis . Involved in recruiting VPS4A and/or VPS4B to the midbody of dividing cells . During late anaphase, involved in nuclear envelope reassembly and mitotic spindle disassembly together with the ESCRT-III complex: IST1 acts by mediating the recruitment of SPAST to the nuclear membrane, leading to microtubule severing . Recruited to the reforming nuclear envelope (NE) during anaphase by LEMD2 . Regulates early endosomal tubulation together with the ESCRT-III complex by mediating the recruitment of SPAST .

Cellular Localization:

Cytoplasmic vesicle . Cytoplasm, cytoskeleton, microtubule organizing center, centrosome . Midbody . Nucleus envelope . Localizes to centrosome and midbody of dividing cells (PubMed:19129480, PubMed:19129479, PubMed:20719964). Colocalized with SPART to the ends of Flemming bodies during cytokinesis (PubMed:20719964). Localizes to the reforming nuclear envelope on chromatin disks during late anaphase. .