Main Information

Target

Histone H2A.Z

Host Species

Rabbit

Reactivity

Human, Mouse, Rat

Applications

WB

MW

14kD (Observed)

Conjugate/Modification

Acetyl

Detailed Information

Recommended Dilution Ratio

WB 1:500-1000

Formulation

PBS, pH 7.4, containing 0.5%BSA, 0.02% sodium azide as Preservative and 50% Glycerol.

Specificity

The antibody detects endogenous Histone H2A.Z (Acetyl Lys7) protein.

Purification

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using specific immunogen.

Storage

-15°C to -25°C/1 year(Do not lower than -25°C)

MW(Observed)

14kD

Modification

Acetyl

Clonality

Polyclonal

Isotype

IgG

Antigen&Target Information

Immunogen:

Synthetic Peptide of Histone H2A.Z (Acetyl Lys7)

Specificity:

The antibody detects endogenous Histone H2A.Z (Acetyl Lys7) protein.

Gene Name:

H2AFZ

Protein Name:

Histone H2A.Z

Other Name:

H2AFZ ;
H2AZ ;
Histone H2A.Z ;
H2A/z

Database Link:

Organism	Gene ID	SwissProt
Human	3015;	P0C0S5;
Mouse	51788;	P0C0S6;
Rat	58940;	P0C0S7;

Background:

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene encodes a replication-independent member of the histone H2A family that is distinct from other members of the family. Studies in mice have shown that this particular histone is required for embryonic development and indicate that lack of functional histone H2A leads to embryonic lethality. [provided by RefSeq, Jul 2008],

Function:

Function:Variant histone H2A which replaces conventional H2A in a subset of nucleosomes. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. May be involved in the formation of constitutive heterochromatin. May be required for chromosome segregation during cell division.,mass spectrometry:Monoisotopic, not modified PubMed:16457589,PTM:Acetylated on Lys-5, Lys-8 and Lys-12 during interphase. Acetylation disappears at mitosis.,PTM:Monoubiquitination of Lys-122 gives a specific tag for epigenetic transcriptional repression.,PTM:Not phosphorylated.,similarity:Belongs to the histone H2A family.,subunit:The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA. H2A or its variant H2AFZ forms an heterodimer with H2B. H2AFZ interacts with INCENP.,

Cellular Localization:

Nucleus. Chromosome.

Tissue Expression:

Brain,Epithelium,Skeletal muscle,Uterus,

Research Areas:

>>Necroptosis ;
>>Neutrophil extracellular trap formation ;
>>Alcoholism ;
>>Systemic lupus erythematosus