Main Information

Target

Lamin B1

Host Species

Mouse

Reactivity

Human, Rat, Mouse

Applications

WB, IHC, IF, IP

Conjugate/Modification

AbFluor 405

Detailed Information

Recommended Dilution Ratio

Optimal working dilutions should be determined experimentally by the investigator; Suggested starting dilutions are as follows:IHC 1:50-300; IF 1:200.

Formulation

Liquid in PBS, pH 7.4, containing 0.02% sodium azide as preservative and 50% Glycerol.

Specificity

Lamin B1 Monoclonal Antibody(7C11) AbFluor™ 405 Conjugated specially designed for your Immunofluorescence analysis.

Purification

The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.

Storage

Stable for one year at -15°C to -25°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing. Store in dark.

Concentration

1mg/ml

Modification

Unmodified

Conjugate

AbFluor 405

Clonality

Monoclonal

Clone Number

7C11

Isotype

IgG

Antigen&Target Information

Specificity:

Lamin B1 Monoclonal Antibody(7C11) AbFluor™ 405 Conjugated specially designed for your Immunofluorescence analysis.

Gene Name:

LMNB1

Protein Name:

Lamin-B1

Other Name:

LMNB1

Database Link:

Organism	Gene ID	SwissProt
Human	4001;	P20700;

Background:

lamin B1(LMNB1) Homo sapiens This gene encodes one of the two B-type lamin proteins and is a component of the nuclear lamina. A duplication of this gene is associated with autosomal dominant adult-onset leukodystrophy (ADLD). Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2015],

Function:

Disease:Defects in LMNB1 are the cause of leukodystrophy demyelinating autosomal dominant adult-onset (ADLD) [MIM:169500]. ADLD is a slowly progressive and fatal demyelinating leukodystrophy, presenting in the fourth or fifth decade of life. Clinically characterized by early autonomic abnormalities, pyramidal and cerebellar dysfunction, and symmetric demyelination of the CNS. It differs from multiple sclerosis and other demyelinating disorders in that neuropathology shows preservation of oligodendroglia in the presence of subtotal demyelination and lack of astrogliosis.,Function:Lamins are components of the nuclear lamina, a fibrous layer on the nucleoplasmic side of the inner nuclear membrane, which is thought to provide a framework for the nuclear envelope and may also interact with chromatin.,miscellaneous:The structural integrity of the lamina is strictly controlled by the cell cycle, as seen by the disintegration and formation of the nuclear envelope in prophase and telophase, respectively.,PTM:B-type lamins undergo a series of modifications, such as farnesylation and phosphorylation. Increased phosphorylation of the lamins occurs before envelope disintegration and probably plays a role in regulating lamin associations.,similarity:Belongs to the intermediate filament family.,subunit:Interacts with lamin-associated polypeptides IA, IB and 2.,

Cellular Localization:

Nucleus lamina .

Tissue Expression:

Brain,Cajal-Retzius cell,Epithelium,Eye,Fetal brain cortex,Ovarian carcinoma,Placenta,Uterus,

Research Areas:

>>Apoptosis