Main Information

Target

COX IV

Host Species

Mouse

Reactivity

Human, Rat, Mouse

Applications

WB, IHC, IF,

MW

20kD (Calculated)

Conjugate/Modification

HRP

Detailed Information

Recommended Dilution Ratio

Optimal working dilutions should be determined experimentally by the investigator; Suggested starting dilutions are as follows:WB 1:1000-3000; IHC 1:50-300.

Formulation

Liquid in PBS, pH 7.4, containing 0.02% sodium azide as preservative and 50% Glycerol.

Specificity

COX IV Monoclonal Antibody(6C8) HRP Conjugated, specially designed for your Western blot analysis.

Purification

The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.

Storage

Stable for one year at -15°C to -25°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Concentration

1mg/ml

MW(Calculated)

20kD

Modification

Unmodified

Conjugate

HRP

Clonality

Monoclonal

Clone Number

6C8

Isotype

IgG1

Antigen&Target Information

Specificity:

COX IV Monoclonal Antibody(6C8) HRP Conjugated, specially designed for your Western blot analysis.

Gene Name:

COX4I1

Protein Name:

Cytochrome c oxidase subunit 4 isoform 1, mitochondrial

Other Name:

COX4I1

Database Link:

Organism	Gene ID	SwissProt
Human	1327;	P13073;

Background:

Cytochrome c oxidase (COX) is the terminal enzyme of the mitochondrial respiratory chain. It is a multi-subunit enzyme complex that couples the transfer of electrons from cytochrome c to molecular oxygen and contributes to a proton electrochemical gradient across the inner mitochondrial membrane. The complex consists of 13 mitochondrial- and nuclear-encoded subunits. The mitochondrially-encoded subunits perform the electron transfer and proton pumping activities. The functions of the nuclear-encoded subunits are unknown but they may play a role in the regulation and assembly of the complex. This gene encodes the nuclear-encoded subunit IV isoform 1 of the human mitochondrial respiratory chain enzyme. It is located at the 3' of the NOC4 (neighbor of COX4) gene in a head-to-head orientation, and shares a promoter with it. Pseudogenes related to this gene are located on chromosomes

Function:

Function:This protein is one of the nuclear-coded polypeptide chains of cytochrome c oxidase, the terminal oxidase in mitochondrial electron transport.,similarity:Belongs to the cytochrome c oxidase IV family.,tissue specificity:Ubiquitous.,

Cellular Localization:

Mitochondrion inner membrane ; Single-pass membrane protein .

Tissue Expression:

Ubiquitous.

Research Areas:

>>Oxidative phosphorylation ;
>>Metabolic pathways ;
>>Cardiac muscle contraction ;
>>Thermogenesis ;
>>Non-alcoholic fatty liver disease ;
>>Alzheimer disease ;
>>Parkinson disease ;
>>Amyotrophic lateral sclerosis ;
>>Huntington disease ;
>>Prion disease ;
>>Pathways of neurodegeneration - multiple diseases ;
>>Chemical carcinogenesis - reactive oxygen species ;
>>Diabetic cardiomyopathy