Main Information

Target

COX IV

Host Species

Mouse

Reactivity

Human, Rat, Mouse

Applications

WB, IHC, IF,

Conjugate/Modification

AbFluor 350

Detailed Information

Recommended Dilution Ratio

Optimal working dilutions should be determined experimentally by the investigator; Suggested starting dilutions are as follows:IHC 1:50-300; IF 1:200 .

Formulation

Liquid in PBS, pH 7.4, containing 0.02% sodium azide as preservative and 50% Glycerol.

Specificity

COX IV Monoclonal Antibody(6C8) AbFluor™ 350 Conjugated specially designed for your Immunofluorescence analysis.

Purification

The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.

Storage

Stable for one year at -15°C to -25°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing. Store in dark.

Concentration

1mg/ml

Modification

Unmodified

Conjugate

AbFluor 350

Clonality

Monoclonal

Clone Number

6C8

Isotype

IgG1

Antigen&Target Information

Specificity:

COX IV Monoclonal Antibody(6C8) AbFluor™ 350 Conjugated specially designed for your Immunofluorescence analysis.

Gene Name:

COX4I1

Protein Name:

Cytochrome c oxidase subunit 4 isoform 1 mitochondrial

Other Name:

COX4I1

Database Link:

Organism	Gene ID	SwissProt
Human	1327;	P13073;

Background:

Cytochrome c oxidase (COX) is the terminal enzyme of the mitochondrial respiratory chain. It is a multi-subunit enzyme complex that couples the transfer of electrons from cytochrome c to molecular oxygen and contributes to a proton electrochemical gradient across the inner mitochondrial membrane. The complex consists of 13 mitochondrial- and nuclear-encoded subunits. The mitochondrially-encoded subunits perform the electron transfer and proton pumping activities. The functions of the nuclear-encoded subunits are unknown but they may play a role in the regulation and assembly of the complex. This gene encodes the nuclear-encoded subunit IV isoform 1 of the human mitochondrial respiratory chain enzyme. It is located at the 3' of the NOC4 (neighbor of COX4) gene in a head-to-head orientation, and shares a promoter with it. Pseudogenes related to this gene are located on chromosomes

Function:

Function:This protein is one of the nuclear-coded polypeptide chains of cytochrome c oxidase, the terminal oxidase in mitochondrial electron transport.,similarity:Belongs to the cytochrome c oxidase IV family.,tissue specificity:Ubiquitous.,

Cellular Localization:

Mitochondrion inner membrane ; Single-pass membrane protein .

Tissue Expression:

Ubiquitous.

Research Areas:

>>Oxidative phosphorylation ;
>>Metabolic pathways ;
>>Cardiac muscle contraction ;
>>Thermogenesis ;
>>Non-alcoholic fatty liver disease ;
>>Alzheimer disease ;
>>Parkinson disease ;
>>Amyotrophic lateral sclerosis ;
>>Huntington disease ;
>>Prion disease ;
>>Pathways of neurodegeneration - multiple diseases ;
>>Chemical carcinogenesis - reactive oxygen species ;
>>Diabetic cardiomyopathy