Main Information

Target

ABCG2

Host Species

Mouse

Reactivity

Human, Mouse, Monkey

Applications

WB, IHC, IF, Flow Cyt, ELISA

MW

72kD (Calculated)

Conjugate/Modification

Unmodified

Detailed Information

Recommended Dilution Ratio

WB 1:500-1:2000; IHC 1:200-1:1000; IF 1:200-1:1000; Flow Cyt 1:200-1:400; ELISA 1:10000; Not yet tested in other applications.

Formulation

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Specificity

ABCG2 Monoclonal Antibody detects endogenous levels of ABCG2 protein.

Purification

Affinity purification

Storage

-15°C to -25°C/1 year(Do not lower than -25°C)

MW(Calculated)

72kD

Modification

Unmodified

Clonality

Monoclonal

Antigen&Target Information

Immunogen:

Purified recombinant fragment of human ABCG2 expressed in E. Coli.

Specificity:

ABCG2 Monoclonal Antibody detects endogenous levels of ABCG2 protein.

Gene Name:

ABCG2

Protein Name:

ATP-binding cassette sub-family G member 2

Other Name:

ABCG2 ;
ABCP ;
BCRP ;
BCRP1 ;
MXR ;
ATP-binding cassette sub-family G member 2 ;
Breast cancer resistance protein ;
CDw338 ;
Mitoxantrone resistance-associated protein ;
Placenta-specific ATP-binding cassette transporter ;
CD antigen CD338

Database Link:

Organism	Gene ID	SwissProt
Human	9429;	Q9UNQ0;
Mouse	26357;	Q7TMS5;

Background:

The membrane-associated protein encoded by this gene is included in the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the White subfamily. Alternatively referred to as a breast cancer resistance protein, this protein functions as a xenobiotic transporter which may play a major role in multi-drug resistance. It likely serves as a cellular defense mechanism in response to mitoxantrone and anthracycline exposure. Significant expression of this protein has been observed in the placenta, which may suggest a potential role for this molecule in placenta tissue. Multiple transcript variants encoding different isoforms have been found for this gene.

Function:

Function:Xenobiotic transporter that may play an important role in the exclusion of xenobiotics from the brain. May be involved in brain-to-blood efflux. Appears to play a major role in the multidrug resistance phenotype of several cancer cell lines. When overexpressed, the transfected cells become resistant to mitoxantrone, daunorubicin and doxorubicin, display diminished intracellular accumulation of daunorubicin, and manifest an ATP-dependent increase in the efflux of rhodamine 123.,induction:Up-regulated in brain tumors.,PTM:Glycosylation-deficient ABCG2 is normally expressed and functional.,similarity:Belongs to the ABC transporter family. ABCG (White) subfamily.,similarity:Contains 1 ABC transmembrane type-2 domain.,similarity:Contains 1 ABC transporter domain.,subunit:Monomer or homodimer; disulfide-linked.,tissue specificity:Highly expressed in placenta. Low expression in small intestine, liver and colon.,

Cellular Localization:

Cell membrane ; Multi-pass membrane protein . Apical cell membrane ; Multi-pass membrane protein . Mitochondrion membrane ; Multi-pass membrane protein . Enriched in membrane lipid rafts. .

Tissue Expression:

Highly expressed in placenta (PubMed:9850061). Low expression in small intestine, liver and colon (PubMed:9861027). Expressed in brain (at protein level) (PubMed:12958161).

Research Areas:

>>Antifolate resistance ;
>>ABC transporters ;
>>Bile secretion