Main Information

Target

DFFB

Host Species

Rabbit

Reactivity

Human, Rat, Mouse,

Applications

IHC, IF, WB

MW

37kD (Observed)

Conjugate/Modification

Unmodified

Detailed Information

Recommended Dilution Ratio

IHC 1:50-200; WB 1:500-2000; IF 1:50-200

Formulation

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Specificity

This antibody detects endogenous levels of human DFFB

Purification

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Storage

-15°C to -25°C/1 year(Do not lower than -25°C)

Concentration

1 mg/ml

MW(Observed)

37kD

Modification

Unmodified

Clonality

Polyclonal

Isotype

IgG

Antigen&Target Information

Immunogen:

Synthesized peptide derived from human DFFB

Specificity:

This antibody detects endogenous levels of human DFFB

Gene Name:

DFFB CAD DFF2 DFF40

Protein Name:

DFFB

Other Name:

DNA fragmentation factor subunit beta ;
Caspase-activated deoxyribonuclease ;
CAD ;
Caspase-activated DNase ;
Caspase-activated nuclease ;
CPAN ;
DNA fragmentation factor 40 kDa subunit ;
DFF-40 ;

Database Link:

Organism	Gene ID	SwissProt
Human	1677;	O76075;

Background:

Apoptosis is a cell death process that removes toxic and/or useless cells during mammalian development. The apoptotic process is accompanied by shrinkage and fragmentation of the cells and nuclei and degradation of the chromosomal DNA into nucleosomal units. DNA fragmentation factor (DFF) is a heterodimeric protein of 40-kD (DFFB) and 45-kD (DFFA) subunits. DFFA is the substrate for caspase-3 and triggers DNA fragmentation during apoptosis. DFF becomes activated when DFFA is cleaved by caspase-3. The cleaved fragments of DFFA dissociate from DFFB, the active component of DFF. DFFB has been found to trigger both DNA fragmentation and chromatin condensation during apoptosis. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene but the biological validity of some of these variants has not been determined. [provided by RefSeq, Sep 2013],

Function:

enzyme regulation:Inhibited by DFFA (DFF45).,Function:Nuclease that induces DNA fragmentation and chromatin condensation during apoptosis. Degrades naked DNA and induces apoptotic morphology.,similarity:Contains 1 CIDE-N domain.,subunit:Heterodimer of DFFA and DFFB.,

Cellular Localization:

Cytoplasm. Nucleus.

Tissue Expression:

Brain,Fetal brain,Lung,Pancreas,

Research Areas:

>>Apoptosis