Main Information

Target

Atg14

Host Species

Rabbit

Reactivity

Human, Mouse, Rat

Applications

IHC, IF

MW

55kD (Observed)

Conjugate/Modification

Unmodified

Detailed Information

Recommended Dilution Ratio

IHC 1:50-300; IF 1:50-200

Formulation

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Specificity

The antibody detects endogenous ATG14L protein

Purification

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Storage

-15°C to -25°C/1 year(Do not lower than -25°C)

Concentration

1 mg/ml

MW(Observed)

55kD

Modification

Unmodified

Clonality

Polyclonal

Isotype

IgG

Antigen&Target Information

Immunogen:

Recombinant Protein of ATG14L

Specificity:

The antibody detects endogenous ATG14L protein

Gene Name:

ATG14 KIAA0831

Protein Name:

Beclin 1-associated autophagy-related key regulator (Barkor) (Autophagy-related protein 14-like protein) (Atg14L)

Other Name:

Beclin 1-associated autophagy-related key regulator ;
Barkor ;
Autophagy-related protein 14-like protein ;
Atg14L ;

Database Link:

Organism	Gene ID	SwissProt
Human	22863;	Q6ZNE5;
Mouse		Q8CDJ3;
Rat		D4A4K3;

Background:

miscellaneous:The sequence shown here is derived from an EMBL/GenBank/DDBJ third party annotation (TPA) entry.,

Function:

miscellaneous:The sequence shown here is derived from an EMBL/GenBank/DDBJ third party annotation (TPA) entry.,

Cellular Localization:

Cytoplasm . Endoplasmic reticulum membrane ; Peripheral membrane protein . Preautophagosomal structure membrane ; Peripheral membrane protein . Cytoplasmic vesicle, autophagosome membrane ; Peripheral membrane protein . Cytosolic under nutrient-rich conditions (PubMed:19050071). Following autophagy stimuli, such as starvation or rapamycin induction, predominantly detected in cytoplasmic foci, identified as isolation membranes and autophagosomes (PubMed:19050071). Accumulates on highly curved PtdIns(3)P enriched autophagic membrane via its BATS domain to sense and maintain membrane curvature (By similarity). Localizes also to discrete punctae along the ciliary axoneme and to the base of the ciliary axoneme (By similarity). .

Tissue Expression:

Brain,Epithelium,Hippocampus,Trachea,

Research Areas:

>>Autophagy - animal ;
>>Alzheimer disease ;
>>Amyotrophic lateral sclerosis ;
>>Huntington disease ;
>>Spinocerebellar ataxia ;
>>Pathways of neurodegeneration - multiple diseases ;
>>Shigellosis ;
>>Kaposi sarcoma-associated herpesvirus infection