Main Information

Target

APG7

Host Species

Rabbit

Reactivity

Human, Mouse, Rat

Applications

WB, IHC, IF

MW

78kD (Observed)

Conjugate/Modification

Unmodified

Detailed Information

Recommended Dilution Ratio

IHC 1:50-300; WB 1:500-2000; IF 1:50-200

Formulation

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Specificity

The antibody detects endogenous ATG7 protein

Purification

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Storage

-15°C to -25°C/1 year(Do not lower than -25°C)

Concentration

1 mg/ml

MW(Observed)

78kD

Modification

Unmodified

Clonality

Polyclonal

Isotype

IgG

Antigen&Target Information

Immunogen:

Recombinant Protein of ATG7

Specificity:

The antibody detects endogenous ATG7 protein

Gene Name:

ATG7 APG7L

Protein Name:

Ubiquitin-like modifier-activating enzyme ATG7 (ATG12-activating enzyme E1 ATG7) (Autophagy-related protein 7) (APG7-like) (hAGP7) (Ubiquitin-activating enzyme E1-like protein)

Other Name:

Ubiquitin-like modifier-activating enzyme ATG7 ;
ATG12-activating enzyme E1 ATG7 ;
Autophagy-related protein 7 ;
APG7-like ;
hAGP7 ;
Ubiquitin-activating enzyme E1-like protein ;

Database Link:

Organism	Gene ID	SwissProt
Human	10533;	O95352;
Mouse		Q9D906;
Rat		Q641Y5;

Background:

This gene encodes an E1-like activating enzyme that is essential for autophagy and cytoplasmic to vacuole transport. The encoded protein is also thought to modulate p53-dependent cell cycle pathways during prolonged metabolic stress. It has been associated with multiple functions, including axon membrane trafficking, axonal homeostasis, mitophagy, adipose differentiation, and hematopoietic stem cell maintenance. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2015],

Function:

Domain:The C-terminal part of the protein is essential for the dimerization and interaction with ATG3 and ATG12.,Function:Functions as an E1 enzyme essential for multisubstrates such as GABARAPL1 and ATG12. Forms intermediate conjugates with GABARAPL1 (GABARAPL2, GABARAP or MAP1ALC3). Formation of the final GABARAPL1-PE conjugate is essential for autophagy.,similarity:Belongs to the ATG7 family.,subunit:Homodimer (By similarity). Interacts with ATG3 and ATG12. The complex, composed of ATG3 and ATG7, plays a role in the conjugation of ATG12 to ATG5.,tissue specificity:Widely expressed, especially in kidney, liver, lymph nodes and bone marrow.,

Cellular Localization:

Cytoplasm . Preautophagosomal structure . Localizes also to discrete punctae along the ciliary axoneme and to the base of the ciliary axoneme. .

Tissue Expression:

Widely expressed, especially in kidney, liver, lymph nodes and bone marrow.

Research Areas:

>>Autophagy - other ;
>>Autophagy - animal ;
>>Ferroptosis ;
>>Neutrophil extracellular trap formation