Main Information

Target

Bmi-1

Host Species

Rabbit

Reactivity

Human

Applications

WB

MW

37-43kD (Observed)

Conjugate/Modification

Unmodified

Detailed Information

Recommended Dilution Ratio

WB 1:500-2000

Formulation

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Specificity

This antibody detects endogenous levels of human Bmi-1

Purification

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Storage

-15°C to -25°C/1 year(Do not lower than -25°C)

Concentration

1 mg/ml

MW(Observed)

37-43kD

Modification

Unmodified

Clonality

Polyclonal

Clone Number

F270

Isotype

IgG

Antigen&Target Information

Immunogen:

Recombinant Protein derived from human Bmi-1

Specificity:

This antibody detects endogenous levels of human Bmi-1

Gene Name:

BMI1 PCGF4 RNF51

Protein Name:

Bmi-1

Other Name:

Polycomb complex protein BMI-1 ;
Polycomb group RING finger protein 4 ;
RING finger protein 51 ;

Database Link:

Organism	Gene ID	SwissProt
Human	100532731;	P35226;

Background:

BMI1 proto-oncogene, polycomb ring finger(BMI1) Homo sapiens This gene encodes a ring finger protein that is major component of the polycomb group complex 1 (PRC1). This complex functions through chromatin remodeling as an essential epigenetic repressor of multiple regulatory genes involved in embryonic development and self-renewal in somatic stem cells. This protein also plays a central role in DNA damage repair. This gene is an oncogene and aberrant expression is associated with numerous cancers and is associated with resistance to certain chemotherapies. A pseudogene of this gene is found on chromosome X. Read-through transcription also exists between this gene and the upstream COMM domain containing 3 (COMMD3) gene. [provided by RefSeq, Sep 2015],

Function:

Disease:Cooperates with the MYC oncogene to produce B-lymphomas.,Function:Component of the Polycomb group (PcG) multiprotein PRC1 complex, a complex required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones; it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility. In the PRC1 complex, it is required to stimulate the E3 ubiquitin-protein ligase activity of RNF2/RING2.,PTM:May be polyubiquitinated; which does not lead to proteasomal degradation.,similarity:Contains 1 RING-type zinc finger.,subunit:Component of chromatin-associated class II polycomb repressive complex 1 (PRC1/hPRC-H) at least composed of PCGF2/RNF110, BMI1/PCGF4, CBX2/M33, CBX4/PC2, CBX8/PC3, PHC1, PHC2, PHC3, SCMH1, RING1 and RNF2/RING2. Also component of E3 ubiquitin ligase complex or human polycomb repressive complex 1-like at least composed of PHC2, BMI1, RING1 and RNF2. Interacts with SPOP. Part of a complex consisting of BMI1, CUL3 and SPOP. Interacts with E4F1.,

Cellular Localization:

Nucleus . Cytoplasm .

Tissue Expression:

Epithelium,Erythrocyte,Muscle,Thymus,

Research Areas:

>>Signaling pathways regulating pluripotency of stem cells ;
>>Transcriptional misregulation in cancer ;
>>MicroRNAs in cancer