Main Information

Target

CaVα2δ2

Host Species

Rabbit

Reactivity

Human, Rat, Mouse

Applications

WB, IHC, IF

MW

100-120kD (Observed)

Conjugate/Modification

Unmodified

Detailed Information

Recommended Dilution Ratio

WB 1:500-1:2000; IHC 1:100-1:300; ELISA 1:40000; IF 1:50-200

Formulation

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Specificity

The antibody detects endogenous CaVα2δ2 protein

Purification

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Storage

-15°C to -25°C/1 year(Do not lower than -25°C)

Concentration

1 mg/ml

MW(Observed)

100-120kD

Modification

Unmodified

Clonality

Polyclonal

Isotype

IgG

Antigen&Target Information

Immunogen:

Synthetic Peptide of CaVα2δ2 AA range: 540-620

Specificity:

The antibody detects endogenous CaVα2δ2 protein

Gene Name:

CACNA2D2

Protein Name:

Voltage-dependent calcium channel subunit alpha-2/delta-2 (Voltage-gated calcium channel subunit alpha-2/delta-2) [Cleaved into: Voltage-dependent calcium channel subunit alpha-2-2; Voltage-dependent calcium channel subunit delta-2]

Other Name:

Voltage-dependent calcium channel subunit alpha-2/delta-2 ;
Voltage-gated calcium channel subunit alpha-2/delta-2 ;
[Cleaved into: Voltage-dependent calcium channel subunit alpha-2-2 ;
Voltage-dependent calcium channel subunit delta-2]

Database Link:

Organism	Gene ID	SwissProt
Human	9254;	Q9NY47;
Mouse		Q6PHS9;
Rat		Q8CFG6;

Background:

calcium voltage-gated channel auxiliary subunit alpha2delta 2(CACNA2D2) Homo sapiens Calcium channels mediate the entry of calcium ions into the cell upon membrane polarization. This gene encodes the alpha-2/delta subunit of the voltage-dependent calcium channel complex. The complex consists of the main channel-forming subunit alpha-1, and auxiliary subunits alpha-2/delta, beta, and gamma. The auxiliary subunits function in the assembly and membrane localization of the complex, and modulate calcium currents and channel activation/inactivation kinetics. The subunit encoded by this gene undergoes post-translational cleavage to yield the extracellular alpha2 peptide and a membrane-anchored delta polypeptide. This subunit is a receptor for the antiepileptic drug, gabapentin. Mutations in this gene are associated with early infantile epileptic encephalopathy. Single nucleotide polymorphisms in this gene are correlated with increased sensitivity to

Function:

Domain:The MIDAS-like motif in the VWFA domain binds divalent metal cations and is required to promote trafficking of the alpha-1 (CACNA1) subunit to the plasma membrane by an integrin-like switch.,Function:The alpha-2/delta subunit of voltage-dependent calcium channels regulates calcium current density and activation/inactivation kinetics of the calcium channel. Acts as a regulatory subunit for P/Q-type calcium channel (CACNA1A), N-type (CACNA1B), L-type (CACNA1C OR CACNA1D) and possibly T-type (CACNA1G). Overexpression induces apoptosis.,miscellaneous:Binds gabapentin, an antiepileptic drug.,PTM:May be proteolytically processed into subunits alpha-2-2 and delta-2 that are disulfide-linked. It is however unclear whether such cleavage really takes place in vivo and has a functional role.,PTM:N-glycosylated.,similarity:Belongs to the calcium channel subunit alpha-2/delta family.,similarity:Contains 1 cache domain.,similarity:Contains 1 VWFA domain.,subcellular location:Colocalizes with CACNA1A in lipid raft fractions.,subunit:Dimer formed of alpha-2-2 and delta-2 chains; disulfide-linked. Voltage-dependent calcium channels are multisubunit complexes, consisting of alpha-1 (CACNA1), alpha-2 (CACNA2D), beta (CACNB) and delta (CACNA2D) subunits in a 1:1:1:1 ratio.,tissue specificity:Predominantly present in cerebellar cortex. Present in various lung tumor cell lines, while it is absent in normal lung (at protein level). Highly expressed in heart, lung, testis, pancreas and skeletal muscle. Also expressed in kidney, liver, placenta and brain.,

Cellular Localization:

Membrane ; Single-pass type I membrane protein . Colocalizes with CACNA1A in lipid raft fractions. .

Tissue Expression:

Predominantly present in cerebellar cortex. Present in various lung tumor cell lines, while it is absent in normal lung (at protein level). Highly expressed in heart, lung, testis, pancreas and skeletal muscle. Also expressed in kidney, liver, placenta and brain.

Research Areas:

>>MAPK signaling pathway ;
>>Cardiac muscle contraction ;
>>Adrenergic signaling in cardiomyocytes ;
>>Oxytocin signaling pathway ;
>>Hypertrophic cardiomyopathy ;
>>Arrhythmogenic right ventricular cardiomyopathy ;
>>Dilated cardiomyopathy