Main Information

Target

V5-Tag

Host Species

Mouse

Reactivity

Species independent

Applications

WB, IP, IF

Conjugate/Modification

Unmodified

Detailed Information

Recommended Dilution Ratio

WB 1:5000; IP 1:200; IF 1:1000

Formulation

PBS, pH 7.4, containing 0.5%BSA, 0.02% sodium azide as Preservative and 50% Glycerol.

Specificity

The antibody detects C-terminal, internal, and N-terminal V5-tag fusion proteins.

Purification

The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.

Storage

-15°C to -25°C/1 year(Do not lower than -25°C)

Modification

Unmodified

Clonality

Monoclonal

Clone Number

3C8

Antigen&Target Information

Immunogen:

Synthetic Peptide of V5-Tag

Specificity:

The antibody detects C-terminal, internal, and N-terminal V5-tag fusion proteins.

Background:

The V5 epitope tag is derived from a small epitope (Pk) present on the P and V proteins of the paramyxovirus of simian virus 5 (SV5). The V5 tag antibody can be helpful in detecting the recombinant proteins, some of which include transmembrane and secreted proteins fusion protein. This V5-tag antibody is prepared by immunizing synthetic peptide GKPIPNPLLGLDST coupled to KLH.