Main Information

Target

GM130

Host Species

Rabbit

Reactivity

Human, Mouse, Rat

Applications

WB, IHC, IF, ELISA

MW

112-130kD (Observed)

Conjugate/Modification

Unmodified

Detailed Information

Recommended Dilution Ratio

WB 1:500-1:2000; ELISA 1:10000; IF 1:50-200

Formulation

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Specificity

This antibody detects endogenous levels of human GM130

Purification

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Storage

-15°C to -25°C/1 year(Do not lower than -25°C)

Concentration

1 mg/ml

MW(Observed)

112-130kD

Modification

Unmodified

Clonality

Polyclonal

Isotype

IgG

Antigen&Target Information

Immunogen:

Synthesized peptide derived from human GM130

Specificity:

This antibody detects endogenous levels of human GM130

Gene Name:

GOLGA2

Protein Name:

GM130

Other Name:

Golgin subfamily A member 2 ;
130 kDa cis-Golgi matrix protein ;
GM130 ;
GM130 autoantigen ;
Golgin-95 ;

Database Link:

Organism	Gene ID	SwissProt
Human	2801;	Q08379;

Background:

The Golgi apparatus, which participates in glycosylation and transport of proteins and lipids in the secretory pathway, consists of a series of stacked cisternae (flattened membrane sacs). Interactions between the Golgi and microtubules are thought to be important for the reorganization of the Golgi after it fragments during mitosis. This gene encodes one of the golgins, a family of proteins localized to the Golgi. This encoded protein has been postulated to play roles in the stacking of Golgi cisternae and in vesicular transport. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of these variants has not been determined. [provided by RefSeq, Feb 2010],

Function:

Domain:Extended rod-like protein with coiled-coil domains.,Function:Golgi auto-antigen; probably involved in maintaining cis-Golgi structure.,sequence Caution:Sequence differs from that shown after position 814 due to an internal deletion.,similarity:Belongs to the GOLGA2 family.,subunit:Part of a larger oligomeric complex. Interacts with p115 (By similarity). Interacts with RAB1B that has been activated by GTP-binding. Interacts with GORASP1/GRASP65 and ZFPL1.,

Cellular Localization:

Golgi apparatus, cis-Golgi network membrane ; Peripheral membrane protein ; Cytoplasmic side . Endoplasmic reticulum-Golgi intermediate compartment membrane ; Peripheral membrane protein ; Cytoplasmic side . Cytoplasm, cytoskeleton, spindle pole . Associates with the mitotic spindle during mitosis (PubMed:26165940). .

Tissue Expression:

Brain,Cervix,Colon adenocarcinoma,Epithelium,Heart,Hepatocyte,Liver,Placenta,Thymus,