Main Information

Target

GAPDH

Host Species

Rabbit

Reactivity

Human, Mouse, Rat, Rabbit, Ch, Mk, sheep, X, Fish, Chicken, Guinea Pig, Duck

Applications

WB, IHC, IF

MW

37kD (Observed)

Conjugate/Modification

Unmodified

Detailed Information

Recommended Dilution Ratio

WB 1:5000; IHC 1:200; IF 1:50-200

Formulation

PBS, pH 7.4, containing 0.5%BSA, 0.02% sodium azide as Preservative and 50% Glycerol.

Specificity

The antibody detects endogenous GAPDH protein.

Purification

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Storage

-15°C to -25°C/1 year(Do not lower than -25°C)

MW(Observed)

37kD

Modification

Unmodified

Clonality

Polyclonal

Isotype

IgG

Antigen&Target Information

Immunogen:

Recombinant Protein of GAPDH

Specificity:

The antibody detects endogenous GAPDH protein.

Gene Name:

GAPDH

Protein Name:

Glyceraldehyde-3-phosphate dehydrogenase

Other Name:

GAPDH ;
GAPD ;
CDABP0047 ;
OK/SW-cl.12 ;
Glyceraldehyde-3-phosphate dehydrogenase ;
GAPDH ;
Peptidyl-cysteine S-nitrosylase GAPDH

Database Link:

Organism	Gene ID	SwissProt
Human	2597;	P04406;
Mouse	100042025;	P16858;
Rat	24383;	P04797;

Background:

glyceraldehyde-3-phosphate dehydrogenase(GAPDH) Homo sapiens This gene encodes a member of the glyceraldehyde-3-phosphate dehydrogenase protein family. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. The product of this gene catalyzes an important energy-yielding step in carbohydrate metabolism, the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD). The encoded protein has additionally been identified to have uracil DNA glycosylase activity in the nucleus. Also, this protein contains a peptide that has antimicrobial activity against E. coli, P. aeruginosa, and C. albicans. Studies of a similar protein in mouse have assigned a variety of additional functions including nitrosylation of nuclear proteins, the regulation of mRNA stability, and acting as a transferri

Function:

Catalytic activity:D-glyceraldehyde 3-phosphate + phosphate + NAD(+) = 3-phospho-D-glyceroyl phosphate + NADH.,Function:Independent of its glycolytic activity it is also involved in membrane trafficking in the early secretory pathway.,online information:Glyceraldehyde 3-phosphate dehydrogenase entry,pathway:Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 1.,pathway:Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 1/5.,PTM:Reversible S-nitrosylation of Cys-152 inhibits enzymatic activity and increases endogenous ADP-ribosylation, which inhibits the enzyme in a non-reversible manner. The latter modification is more likely to be a pathophysiological event associated with inhibition of gluconeogenesis.,sequence Caution:Differs quite extensively.,similarity:Belongs to the glyceraldehyde-3-phosphate dehydrogenase family.,subcellular location:Postnuclear and Perinuclear regions.,subunit:Homotetramer.,subunit:Homotetramer. Binds PRKCI.,

Cellular Localization:

Cytoplasm, cytosol . Nucleus . Cytoplasm, perinuclear region . Membrane . Cytoplasm, cytoskeleton . Translocates to the nucleus following S-nitrosylation and interaction with SIAH1, which contains a nuclear localization signal (By similarity). Postnuclear and Perinuclear regions (PubMed:12829261). .

Tissue Expression:

Astrocytoma,Brain,Cajal-Retzius cell,Colon adenocarcinoma,Epitheliu

Research Areas:

>>Glycolysis / Gluconeogenesis ;
>>Metabolic pathways ;
>>Carbon metabolism ;
>>Biosynthesis of amino acids ;
>>HIF-1 signaling pathway ;
>>Alzheimer disease ;
>>Pathogenic Escherichia coli infection ;
>>Salmonella infection ;
>>Diabetic cardiomyopathy