Main Information

Target

HSP70

Host Species

Mouse

Reactivity

Human, Mouse, Rat

Applications

WB, IHC, IF

MW

70kD (Observed)

Conjugate/Modification

Unmodified

Detailed Information

Recommended Dilution Ratio

WB 1:1000-2000; IF 1:100-200; IHC 1:50-300

Formulation

PBS, pH 7.4, containing 0.5%BSA, 0.02% sodium azide as Preservative and 50% Glycerol.

Specificity

The antibody detects endogenous HSP70 proteins.

Purification

The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.

Storage

-15°C to -25°C/1 year(Do not lower than -25°C)

MW(Observed)

70kD

Modification

Unmodified

Clonality

Monoclonal

Clone Number

3G10

Antigen&Target Information

Immunogen:

Human protein HSP70 at C-terminal

Specificity:

The antibody detects endogenous HSP70 proteins.

Gene Name:

HSPA1A;HSPA1B

Protein Name:

Heat shock 70 kDa protein 1A/1B

Other Name:

HSPA1A ;
HSPA1 ;
HSPA1B ;
Heat shock 70 kDa protein 1A/1B ;
Heat shock 70 kDa protein 1/2 ;
HSP70-1/HSP70-2 ;
HSP70.1/HSP70.2

Database Link:

Organism	Gene ID	SwissProt
Human	3303;3304;	P0DMV8;P0DMV9;

Background:

This intronless gene encodes a 70kDa heat shock protein which is a member of the heat shock protein 70 family. In conjuction with other heat shock proteins, this protein stabilizes existing proteins against aggregation and mediates the folding of newly translated proteins in the cytosol and in organelles. It is also involved in the ubiquitin-proteasome pathway through interaction with the AU-rich element RNA-binding protein 1. The gene is located in the major histocompatibility complex class III region, in a cluster with two closely related genes which encode similar proteins. [provided by RefSeq, Jul 2008],

Function:

Function:In cooperation with other chaperones, Hsp70s stabilize preexistent proteins against aggregation and mediate the folding of newly translated polypeptides in the cytosol as well as within organelles. These chaperones participate in all these processes through their ability to recognize nonnative conformations of other proteins. They bind extended peptide segments with a net hydrophobic character exposed by polypeptides during translation and membrane translocation, or following stress-induced damage. In case of rotavirus A infection, serves as a post-attachment receptor for the virus to facilitate entry into the cell.,induction:By heat shock.,similarity:Belongs to the heat shock protein 70 family.,subunit:HSPA1B is found in a sperm-specific complex with CATSPER1 and CATSPERB (By similarity). Interacts with TSC2. Interacts with IRAK1BP1.,tissue specificity:HSPA1B is testis-specific.,

Cellular Localization:

Cytoplasm . Nucleus . Cytoplasm, cytoskeleton, microtubule organizing center, centrosome . Secreted . Localized in cytoplasmic mRNP granules containing untranslated mRNAs.

Tissue Expression:

Brain,Cajal-Retzius cell,Embryonic kidney,Epithelium,Fetal

Research Areas:

>>Spliceosome ;
>>MAPK signaling pathway ;
>>Protein processing in endoplasmic reticulum ;
>>Endocytosis ;
>>Longevity regulating pathway - multiple species ;
>>Antigen processing and presentation ;
>>Estrogen signaling pathway ;
>>Prion disease ;
>>Legionellosis ;
>>Toxoplasmosis ;
>>Measles ;
>>Lipid and atherosclerosis