产品简介：
MC1061/P3 Chemically Competent E. coli are used for highly efficient transformations that require the P3 episome for selection and maintenance of vectors encoding the tyrosine tRNA suppressor (synthetic supF gene, such as pCDM8, pcDNA1.1, or any other supF-containing vector).

MC1061 E. coli cells were used by Hanahan and coworkers to engineer the DH10B strain. Among the replaced genes by adding mutations were recA1 (inhibiting the homologous recombination system), endA1, and lacZΔM15. Thus, this strain with its intact homologous recombination system is used in applications where recombination is needed, such as in genetically engineering E. coli by recombination or plasmid-phage recombination.

 

产品参数：
出品公司：MoBiTec GmbH
抗性：Streptomycin（链霉素，工作浓度建议为 50ug/ml）
菌株类型：E.coli
基因型：F–hsdR(rK–, mK+) araD139 Δ(araABC-leu)7679 galU galK ΔlacX74 rpsL(StrR) thi mcrB / P3: KanR AmpR (am) TetR (am)

培养条件：37℃，有氧，LB
质粒转化：42℃热激
保存方式：-20℃或-80℃
备注：MC1061感受态E. coli，用于需要P3附加体才能选择和维持的载体的高效率转化（例如，pCDM8、pcDNA™1.1或任何一种包含supF的载体）。

 

注意事项：
1、为了您的安全和健康，请穿实验服并戴一次性手套操作。本产品仅限于专业人员的科学研究用，不得用于临床诊断或治疗等其他用途。
2、使用甘油菌可不完全融解，在菌液表面蘸取少量涂布固体琼脂平板即可。完全融解后使用，菌体会逐渐沉淀至管底，请混匀后使用。随着冻融次数的增加，菌株的活力会逐渐下降。