产品简介：
Molecular chaperones have been demonstrated to be involved in the protein folding process, and numerous studies have been conducted to elucidate the mechanisms of in vivo protein folding. Takara's Chaperone Plasmid Set consists of five different types of "chaperone team" plasmids are designed to enable efficient expression of multiple molecular chaperones known to work in cooperation in the folding process. It has been reported that coexpression of a target protein with one of these chaperone teams increases recovery of expressed proteins in the soluble fraction. Such proteins are often unrecoverable using conventional methods due to the formation of inclusion bodies.

 

产品参数：
出品公司：Takara
克隆方法：多克隆位点，限制性内切酶
启动子：araB
载体大小：5kb
载体抗性：chloromycetin (氯霉素)
诱导方式：阿拉伯糖
分子伴侣：tig
备注: 伴侣分子表达质粒

 

 

 

 

注意事项：
1、为了您的安全和健康，请穿实验服并戴一次性手套操作。本产品仅限于专业人员的科学研究用，不得用于临床诊断或治疗等其他用途。
2、使用甘油菌可不完全融解，在菌液表面蘸取少量涂布固体琼脂平板即可。完全融解后使用，菌体会逐渐沉淀至管底，请混匀后使用。随着冻融次数的增加，菌株的活力会逐渐下降。 

质粒序列：

CGTATGGCAATGAAAGACGGTGAGCTGGTGATATGGGATAGTGTTCACCCTTGTTACACCGTTTTCCATGAGCAAACTGAAACGTTTTCATCGCT

CTGGAGTGAATACCACGACGATTTCCGGCAGTTTCTACACATATATTCGCAAGATGTGGCGTGTTACGGTGAAAACCTGGCCTATTTCCCTAAAG

GGTTTATTGAGAATATGTTTTTCGTCTCAGCCAATCCCTGGGTGAGTTTCACCAGTTTTGATTTAAACGTGGCCAATATGGACAACTTCTTCGCCC

CCGTTTTCACCATGGGCAAATATTATACGCAAGGCGACAAGGTGCTGATGCCGCTGGCGATTCAGGTTCATCATGCCGTCTGTGATGGCTTCCAT

GTCGGCAGAATGCTTAATGAATTACAACAGTACTGCGATGAGTGGCAGGGCGGGGCGTAATTTTTTTAAGGCAGTTATTGGTGCCCTTAAACGC

CTGGTGCTACGCCTGAATAAGTGATAATAAGCGGATGAATGGCAGAAATTCGAAGAATAGTTACGGCTTATGACATCTTTGTGGACACATCATTC

ACTTTTTATTCACATCCGGCCCTGAACTCGCTAGGACTTGCCCCGGTGCATTTTTTAAATACCCGCGAAAAATAGAGCTGATCGTCAAATCCAACA

TTGCGCCCAACGGTCGCTATCGGCATTCGCGTAGTGCTAAGCAGAAGTTTCGCCTGGCTGATACGCTGATCTTCGCGCCAGCTCAATACGCTAAT

GCCTAACTGCTGGCGGAACAGATGTGATAACCGGGAGGGCGACAGGCAGACATGCTGGGCGACGCTGGCGATATCAAAATGGCTGTCCGCCAGA

TGGTCGCTGATATACTGGCAGGCATCGCGCACACGGCTATCCATCGGCGGGTGCAACGACTCATTAATTACCGCCATACGTCTGAGCAACAACTG

CTCCAGCAGATTGATCGCCAGTAGCTCAGAATAGCGACCTTCCCCTTGCCCGGCGCTGATGATCTGCCCGAACAGTTCGCTGAAATGCGGCTGGC

GCGCCTCGTCCGGGCGGAAAAATCCTGTCTGGGCAAAGATTGTCGGCCAGGTCAGCCACTCCTGCCAGTAGGCGCGAGGCCGGAAATAAACCCA

CTGGTGATACCACTCGCTGGCGTCCGGATGCCGTCCATAGTGATGAATCTCGCCCGGCGGAAACAATAATATATCGCCAGGCCGACAGACAAACT

GCTCGCCATTATTATTAATGACGCCCTCTCCGCGGATGGTCAGGTTAAGAATATATCCCTTCATGCCCAACGGACGATCGATAAAAAAATCCAGAT

ATCCATTCGCTTCAATTGGCGTCAGCCCGGCGACCAGATGGGCATTAAATGAATATCCCGGCAATAGCGGATCATTTTGCGTTTCAGCCATGATT

TCTCTACCCCCCGATGTTCAGAGAAGAAACAAATTGTCCATATCGACCAGGACGACAGAGCTTCCGTCTCCGCAAGACTTTGCGCTTGATGAAAG

CACGTATCAACCCCGCTTGTGAAAAGCGCTTTGTAACAAAAGCGTACAGTTCAGGCGATAAAATTAAGTAACAGAAGTGTCTATAACTATGGCTGG

AATGTCCACATTGAATATTTGCACAGCGTCACACTTTGCAAAGCATTAGCATTTTTGTCCATAAGATTAGCGGATCCTGCCTGACGGTTTTTGCCG

CGACTCTCTATAATTTCTCCATACCTGTTTTTCTGGATGGAGTAAGACCATGGCCTTTCGGGATGATTCTGGTAACAGGGAATGTGATTGATTATA

AGAACATCCCGGTTCCGCGAAGCCAACAACCTGTGCTTGCGGGGTAAGAGTTGACCGAGCACTGTGATTTTTTGAGGTAACAAGATGCAAGTTTC

AGTTGAAACCACTCAAGGCCTTGGCCGCCGTGTAACGATTACTATCGCTGCTGACAGCATCGAGACCGCTGTTAAAAGCGAGCTGGTCAACGTTG

CGAAAAAAGTACGTATTGACGGCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCGCGTCTGTACGCCAGGACGTTCTG

GGTGACCTGATGAGCCGTAACTTCATTGACGCCATCATTAAAGAAAAAATCAATCCGGCTGGCGCACCGACTTATGTTCCGGGCGAATACAAGCT

GGGTGAAGACTTCACTTACTCTGTAGAGTTTGAAGTTTATCCGGAAGTTGAACTGCAGGGTCTGGAAGCGATCGAAGTTGAAAAACCGATCGTTG

AAGTGACCGACGCTGACGTTGACGGCATGCTGGATACTCTGCGTAAACAGCAGGCGACCTGGAAAGAAAAAGACGGCGCTGTTGAAGCAGAAGA

CCGCGTAACCATCGACTTCACCGGTTCTGTAGACGGCGAAGAGTTCGAAGGCGGTAAAGCGTCTGATTTCGTACTGGCGATGGGCCAGGGTCGT

ATGATCCCGGGCTTTGAAGACGGTATCAAAGGCCACAAAGCTGGCGAAGAGTTCACCATCGACGTGACCTTCCCGGAAGAATACCACGCAGAAA

ACCTGAAAGGTAAAGCAGCGAAATTCGCTATCAACCTGAAGAAAGTTGAAGAGCGTGAACTGCCGGAACTGACTGCAGAATTCATCAAACGTTT

CGGCGTTGAAGATGGTTCCGTAGAAGGTCTGCGCGCTGAAGTGCGTAAAAACATGGAGCGCGAGCTGAAGAGCGCCATCCGTAACCGCGTTA

AGTCTCAGGCGATCGAAGGTCTGGTAAAAGCTAACGACATCGACGTACCGGCTGCGCTGATCGACAGCGAAATCGACGTTCTGCGTCGCCAGGCT

GCACAGCGTTTCGGTGGCAACGAAAAACAAGCTCTGGAACTGCCGCGCGAACTGTTCGAAGAACAGGCTAAACGCCGCGTAGTTGTTGGCCTGC

TGCTGGGCGAAGTTATCCGCACCAACGAGCTGAAAGCTGACGAAGAGCGCGTGAAAGGCCTGATCGAAGAGATGGCTTCTGCGTACGAAGAT

CCGAAAGAAGTTATCGAGTTCTACAGCAAAAACAAAGAACTGATGGACAACATGCGCAATGTTGCTCTGGAAGAACAGGCTGTTGAAGCTGTACT

GGCGAAAGCGAAAGTGACTGAAAAAGAAACCACTTTCAACGAGCTGATGAACCAGCAGGCGTAATTTACGCAGCATAACGCGCTAAATTCGCAC

AAAGGCCCGTCACCGCCAGGTGGTGGGCTTTTTTTTGTCATGAATTTTGCATGGAACCGTGCGAAAAGCCTCTTTCGGTGTTAGCGTAACAACA

AAAGATTGTTATGCTTGAAATATGGTGATGCCGTACCCATAACACAGGGACTAGCTGATAATCCGTCCATAAGGTTACAATCGGTACAGCAGGT

TTTTTCAATTTTATCCAGGAGACGGAAATGTCATACAGCGGCGAACGAGATAACTTTGCACCCCATAGATCTGGTACCAAGCTTATCGATGATAA

GCTGTCAAACATGAGAATTACAACTTATATCGTATGGGGCTGACTTCAGGTGCTACATTTGAAGAGATAAATTGCACTGAAATCTAGAAATATTT

TATCTGATTAATAAGATGATCTTCTTGAGATCGTTTTGGTCTGCGCGTAATCTCTTGCTCTGAAAACGAAAAAACCGCCTTGCAGGGCGGTTTTT

CGAAGGTTCTCTGAGCTACCAACTCTTTGAACCGAGGTAACTGGCTTGGAGGAGCGCAGTCACCAAAACTTGTCCTTTCAGTTTAGCCTTAACC

GGCGCATGACTTCAAGACTAACTCCTCTAAATCAATTACCAGTGGCTGCTGCCAGTGGTGCTTTTGCATGTCTTTCCGGGTTGGACTCAAGACG

ATAGTTACCGGATAAGGCGCAGCGGTCGGACTGAACGGGGGGTTCGTGCATACAGTCCAGCTTGGAGCGAACTGCCTACCCGGAACTGAGTG

TCAGGCGTGGAATGAGACAAACGCGGCCATAACAGCGGAATGACACCGGTAAACCGAAAGGCAGGAACAGGAGAGCGCACGAGGGAGCCGCC

AGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCACTGATTTGAGCGTCAGATTTCGTGATGCTTGTCAGGGGGGCGGAGC

CTATGGAAAAACGGCTTTGCCGCGGCCCTCTCACTTCCCTGTTAAGTATCTTCCTGGCATCTTCCAGGAAATCTCCGCCCCGTTCGTAAGCCAT

TTCCGCTCGCCGCAGTCGAACGACCGAGCGTAGCGAGTCAGTGAGCGAGGAAGCGGAATATATCCTGTATCACATATTCTGCTGACGCACCGG

TGCAGCCTTTTTTCTCCTGCCACATGAAGCACTTCACTGACACCCTCATCAGTGCCAACATAGTAAGCCAGTATACACTCCGCTAGCGCTGATG

TCCGGCGGTGCTTTTGCCGTTACGCACCACCCCGTCAGTAGCTGAACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAA

ACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCCGACGCACTTTGCGCCGAATAAATACCTGTGACGGAAGATCACTTCGCAGAATA

AATAAATCCTGGTGTCCCTGTTGATACCGGGAAGCCCTGGGCCAACTTTTGGCGAAAATGAGACGTTGATCGGCACGTAAGAGGTTCCAACTT

TCACCATAATGAAATAAGATCACTACCGGGCGTATTTTTTGAGTTATCGAGATTTTCAGGAGCTAAGGAAGCTAAAATGGAGAAAAAAATCACT

GGATATACCACCGTTGATATATCCCAATGGCATCGTAAAGAACATTTTGAGGCATTTCAGTCAGTTGCTCAATGTACCTATAACCAGACCGTTC

AGCTGGATATTACGGCCTTTTTAAAGACCGTAAAGAAAAATAAGCACAAGTTTTATCCGGCCTTTATTCACATTCTTGCCCGCCTGATGAATGC

TCATCCGGAATTC