产品简介：
pTWIN1 is an E. coli plasmid cloning vector designed for recombinant protein expression, labeling, and cyclization using the IMPACT-TWIN Kit. It contains the pMB1 origin of replication from pBR322 and is maintained at a similar copy number to pBR322; in addition, pTWIN1 also contains an M13 origin of replication. The multiple cloning site (MCS) is positioned to allow translational fusion of an intein tag to the N-terminus, C-terminus, or both, of the cloned target protein. The mini-inteins encoded by pTWIN1, the Ssp DnaB intein and the Mxe GyrA intein, cleave the peptide bond at their C- and N-termini, respectively. The chitin binding domain (CBD) from B.circulans, fused to each intein, facilitates purification of the intein-target protein precursor. Transcription of the intein tags is controlled by the inducible T7 promoter, requiring E. coli strains containing integrated copies of the T7 RNA polymerase gene for expression.

 

产品参数：

质粒类型：大肠杆菌表达载体

出品公司：NEB
克隆方法：多克隆位点，限制性内切酶

启动子：T7

载体大小：7375 bp

检测标签：无

载体抗性：Ampicillin (氨苄青霉素)
诱导方式: IPTG或乳糖及其类似物

 

 

 

 

 

 

注意事项：
1、为了您的安全和健康，请穿实验服并戴一次性手套操作。本产品仅限于专业人员的科学研究用，不得用于临床诊断或治疗等其他用途。
2、使用甘油菌可不完全融解，在菌液表面蘸取少量涂布固体琼脂平板即可。完全融解后使用，菌体会逐渐沉淀至管底，请混匀后使用。随着冻融次数的增加，菌株的活力会逐渐下降。