产品简介:
BL21-Gold(DE3) competent cells is improved version of BL21 competent cells. These high-performance competent cells provide increased transformation efficiency and produce high-quality miniprep DNA. This BL21-Gold-derived expression strain is ideal for performing protein expression studies that utilize the T7 RNA polymerase promoter to direct high-level expression. Derived from Escherichia coli B, this expression strain naturally lack the Lon protease, which can degraderecombinant proteins. In addition, these strains are engineered to be deficient for a second protease, the OmpT protein.
产品参数:
出品公司:Stratagene
抗性:Tetracycline
载体类型:原核表达载体
基因型:
菌株类型:E.coli
培养条件:37℃,有氧,LB
质粒转化:42℃热激
保存方式:20%甘油,-20℃
应用:可以同时用作蛋白表达菌株和质粒克隆菌株
注意事项:
1、为了您的安全和健康,请穿实验服并戴一次性手套操作。本产品仅限于专业人员的科学研究用,不得用于临床诊断或治疗等其他用途。
2、使用甘油菌可不完全融解,在菌液表面蘸取少量涂布固体琼脂平板即可。完全融解后使用,菌体会逐渐沉淀至管底,请混匀后使用。随着冻融次数的增加,菌株的活力会逐渐下降。