产品简介：
pMAL-c4X is an E. coli plasmid cloning vector designed for recombinant protein expression and purification using the pMAL Protein Fusion and Purification System. It contains the pMB1 origin of replication from pBR322 and is maintained at a similar copy number to pBR322; in addition, pMAL-c4X also contains an M13 origin of replication. The multiple cloning site (MCS) is positioned to allow translational fusion of the E. coli maltose binding protein (MBP, encoded by the malE gene) to the N-terminus of the cloned target protein. MBP’s affinity for amylose allows easy purification of the fusion protein, and the MBP domain can be subsequently removed using Factor Xa protease. Cloning of the target gene at the MCS disrupts expression of lacZα, allowing for insert screening by α-complementation. pMAL-c4-series vectors are identical to the pMAL-p4-series vectors above except for a deletion of the malE signal sequence.

 

产品参数：

质粒类型：大肠杆菌表达载体

出品公司：NEB

表达水平：高

克隆方法：多克隆位点，限制性内切酶

载体大小：6645 bp

5' 测序引物及序列：MalE引物: 5'-GGTCGTCAGACTGTCGATGAAGCC-3';
MBP-F: 5'-gatgaagccctgaaagacgcgcag-3'
3' 测序引物及序列：pBad-R: 5'-gatttaatctgtatcagg-3';
M13-F: 5'-TGTAAAACGACGGCCAGT-3'
载体标签：N-MBP, N-Factor X
载体抗性：Ampicillin (氨苄青霉素)
备注：融合表达麦芽糖结合蛋白MBP，蛋白定位于细胞周质

 

 

注意事项：
1、为了您的安全和健康，请穿实验服并戴一次性手套操作。本产品仅限于专业人员的科学研究用，不得用于临床诊断或治疗等其他用途。
2、使用甘油菌可不完全融解，在菌液表面蘸取少量涂布固体琼脂平板即可。完全融解后使用，菌体会逐渐沉淀至管底，请混匀后使用。随着冻融次数的增加，菌株的活力会逐渐下降。