pGL3-Control Vector

The pGL3-Control Vector contains SV40 promoter and enhancer sequences,

resulting in strong expression of luc+ in many types of mammalian cells. This

plasmid is useful in monitoring transfection efficiency, in general, and is a

convenient internal standard for promoter and enhancer activities expressed

by pGL3 recombinants.

pGL3-Control Vector Sequence Reference Points:

Multiple cloning region  1–41

Promoter  48–250

Luciferase gene (luc+)  280–1932

GLprimer2 binding site  281–303

SV40 late poly(A) signal  1964–2185

Enhancer  2205–2441

RVprimer4 binding site  2499–2518

ColE1-derived plasmid replication origin  2756

β-lactamase gene (Ampr)  3518–4378

f1 origin  4510–4965

upstream poly(A) signal  5096–5249

RVprimer3 binding site  5198–5217