产品简介：
The pET-49b(+) vector carries N-terminal GST•Tag™ and His•Tag? coding sequences followed by a recognition site for the human rhinovirus (HRV) 3C protease. This protease is highly specifi c for cleavage of the sequence LEVLFQ↓GP , and is active at low temperatures . pET-49b(+) also contains an optional C-terminal thrombin recognition site followed by an S•Tag™ coding sequence. Unique restriction .sites are shown on the circle map. Note that the sequence is numbered by the pBR322 convention, so the T7 expression region is reversed on the circle map. The cloning/expression region of the coding strand transcribed by T7 RNA polymerase is shown below. The f1 origin is oriented so that infection with the helper phage will produce virions containing single-stranded DNA that corresponds to the coding strand. Therefore, single-stranded sequencing should be performed using the AS S•Tag 18mer Primer 。

 

产品参数：
别名：pET49b, pET 49b
质粒类型：大肠杆菌表达载体
出品公司：Novagen
表达水平：高
克隆方法：多克隆位点，限制性内切酶
载体大小：5926 bp
5' 测序引物：T7或者pGEX 5'
5' 测序引物序列：T7:5'-TAATACGACTCACTATAGGG-3'; pGEX 5':5'-GGGCTGGCAAGCCACGTTTGGTG-3'
3' 测序引物：T7t
3' 测序引物序列：T7t:5'-TGCTAGTTATTGCTCAGCGG-3'
载体标签：N-GST, N-His, N-HRV 3C, C-S, C-Thrombin
载体抗性：Kanamycin (卡那霉素)

注意事项：
1、为了您的安全和健康，请穿实验服并戴一次性手套操作。本产品仅限于专业人员的科学研究用，不得用于临床诊断或治疗等其他用途。
2、使用甘油菌可不完全融解，在菌液表面蘸取少量涂布固体琼脂平板即可。完全融解后使用，菌体会逐渐沉淀至管底，请混匀后使用。随着冻融次数的增加，菌株的活力会逐渐下降。