产品简介：

Cellulose binding domains(CBDs) occur in nature as discrete regions of cellulases and cellulosome scaffolding proteins. Cellulose binding domains provide a new class of affinity tags with many hiahly desirable attributes, including:

1. a readily available,low cost affinity matrix (cellulose) with inherent low non-specific protein binding characteristics.

2.high affinity binding that facilitates rapid purification from dilute and/or complex samples.

3.efficient release under nondenaturing conditions,and.

4.effective immobilization of CBD fusion proteins to cellulose-based matrices without the need for covalent crosslinking.

Then pET-35b(+) vector encodes the CBD clos domain and direct expression to the E.coli cytoplasm.

 

产品参数：

质粒类型：原核表达载体

出品公司：Novagen

启动子：T7-lac

克隆方法：多克隆位点，限制性内切酶

5' 测序引物及序列：T7

3' 测序引物及序列：5'-TAATACGACTCACTATAGGG-3'

载体标签：N-CBDclos,C-His

载体抗性：Kanamycin(卡那霉素)

注意事项：
1、为了您的安全和健康，请穿实验服并戴一次性手套操作。本产品仅限于专业人员的科学研究用，不得用于临床诊断或治疗等其他用途。
2、使用甘油菌可不完全融解，在菌液表面蘸取少量涂布固体琼脂平板即可。完全融解后使用，菌体会逐渐沉淀至管底，请混匀后使用。随着冻融次数的增加，菌株的活力会逐渐下降。