产品简介：

The pET-31b(+) vector is designed for cloning and high-level expression of peptide sequences fused with the 125aa ketosteroid isomerase protein.The unique AlwN I cloning site allows the unidirectional insertion of of tandemly repeated peptide coding regions separated by methionine codons. Unique sites are shown on the circle map. Note that the sequence is numbered by the pBR322 convention, so the T7 expression region is reversed on the circular map. The cloning/expression region of the coding strand transcribed by T7 RNA polymerase is shown below. The f1 origin is oriented so that infection with helper phage will produce virions containing single-stranded DNA that corresponds to the coding strand. Therefore, single-stranded sequencing should be performed using the T7 terminator primer.

 

产品参数：

别名：pET31b, pet 31b

质粒类型：原核表达载体

出品公司：Novagen

表达水平：高

克隆方法：多克隆位点，限制性内切酶

载体大小：5742 bp

5' 测序引物：T7

5' 测序引物序列：5'-TAATACGACTCACTATAGGG-3'

载体标签：C-His

载体抗性：Ampicillin (氨苄青霉素)

备注：Fuse with ketosteroid isomerase for high yield bioproduction of peptides and small proteins; unique AlwNI cloning site for unidirectional insertion of tandemly repeated peptides.

注意事项：
1、为了您的安全和健康，请穿实验服并戴一次性手套操作。本产品仅限于专业人员的科学研究用，不得用于临床诊断或治疗等其他用途。
2、使用甘油菌可不完全融解，在菌液表面蘸取少量涂布固体琼脂平板即可。完全融解后使用，菌体会逐渐沉淀至管底，请混匀后使用。随着冻融次数的增加，菌株的活力会逐渐下降。