产品简介:
The pET-17b vector carries an N-terminal 11aa T7 tag sequence followed by a region of useful cloning sites. Included in the multiple cloning region are dual BstX I sites, which allow efficient cloning using an asymmetric linker. Unique sites (except for the two BstX I sites) are shown on the circle map. Note that the sequence is numbered by the pBR322 convention, so the T7 expression region is reversed on the circluar map. The cloning/expression region of the coding strand transcribed by T7 RNA polymerase is shown below.
产品参数:
载体别名:pET17b, pet 17b,pET-17b(+)
质粒类型:大肠杆菌表达载体
出品公司:Novagen
表达水平:高
克隆方法:多克隆位点,限制性内切酶
载体大小:3306bp
5' 测序引物:T7
3' 测序引物:T7-ter
载体标签:N-T7
载体抗性:Ampicillin (氨苄青霉素)
注意事项:
1、为了您的安全和健康,请穿实验服并戴一次性手套操作。本产品仅限于专业人员的科学研究用,不得用于临床诊断或治疗等其他用途。
2、使用甘油菌可不完全融解,在菌液表面蘸取少量涂布固体琼脂平板即可。完全融解后使用,菌体会逐渐沉淀至管底,请混匀后使用。随着冻融次数的增加,菌株的活力会逐渐下降。