pShuttle-IRES-hrGFP-1 载体

质粒类型:    腺病毒系统

高拷贝/低拷贝:    低拷贝

启动子:    CMV

克隆方法:    多克隆位点，限制性内切酶

载体大小:    8.9 kb

5' 测序引物及序列:    pShuttle-IRES-hrGFP-1-F: CTCACGGGGATTTCCAAGTC

3' 测序引物及序列:    pShuttle-IRES-hrGFP-1-R: ATGCAGTCGTCGAGGAATTG

载体抗性:    Kanamycin (卡那霉素)

备注:    载体内包含有IRES序列和hrGFP序列

载体描述

Two additional shuttle vectors, pShuttle-IRES-hrGFP-1 (Catalog #240081) and pShuttle-IRES-hrGFP-2 (Catalog #240082) are available separately. Both of these vectors contain the CMV promoter and a dicistronic expression cassette in which the multiple cloning site (MCS) is followed by the EMCV-IRES, which directs translation of a humanized recombinant green fluorescent protein (hrGFP) from a novel marine organism as a second open reading frame. This design allows the expression of the gene of interest (up to 5.2 kb) to be monitored at the single-cell level due to expression of the hrGFP on the same transcript. The gene of interest may be fused to three contiguous copies of either the FLAG® epitope (pShuttle-IRES-hrGFP-1) or the HA epitope (pShuttle-IRES-hrGFP-2).