产品简介：

The plasmid pAdEasy-1,containing most of the human adenovirus serotype 5 genome,is deleted for the genes E1 and E3.The removal of these two viral genes creates space for

foreian DNA and eliminates selfreplication capabilities.The deletion of both genes creates room for up to 7.5 kb of foreign DNA that can be inserted into the Ad5 genome.The E1 gene,which is necessary for production of viral particles,is provided in trans by AD-293 cells. pAdEasy-1 carries the ampicillin resistance gene,which is lost after recombination with a shuttle vector.

Recombine plasmids from the shuttle vectors (pShuttle, pShuttle-CMV, pAdTrack, pAdTrack-CMV) into the adenoviral plasmid pAdEasy?-1.

Plasmids recombined in pAdEasy-1 should be packaged in either 293 or 911 cells to make virus.

 

 

产品参数：

质粒类型：腺病毒系统

出品公司：Stratagene

高拷贝/低拷贝：低拷贝

启动子：CMV

克隆方法：多克隆位点，限制性内切酶

载体大小：33441 bp

载体抗性：Ampicillin (氨苄青霉素)