载体简介：

pRNATin-H1.2/Retro siRNA expression vector is compatible with Clontech Retro-X Expression System. It uses an inducible H1 promoter for siRNA expression. The promoter contains the tetracycline operator (TetO1). As a competitor, tetracycline or doxycycline can bind this operator to remove the blockade of tetracycline repressor (TetR) from H1 promoter and induce the transcription of siRNA.

The vector also carries a neomycin resistance gene for establishing stable transfection cell line and a cGFP marker for tracking transfection efficiency, both genes are under the control of SV40 promoter. The vector uses CMV enhancer/promoter joined MSV 5’LTR (CMV/MSV 5'LTR) and MSV 3'LTR for viral transcription and packaging.

产品参数：

出品公司：Genscript.

质粒类型:RNAi载体

启动子:H1.2

克隆方法:多克隆位点，限制性内切酶

载体大小:7706 bp

5' 测序引物及序列:CCAACTTGTTTATTGCAGCT

3' 测序引物及序列:TAATACGACTCACTATAGGG

载体标签:cGFP

载体抗性:Ampicillin (氨苄青霉素)

筛选标记:Neomycin (新霉素)