产品简介：

pRevTRE is a retroviral Tet response vector that expresses a gene of interest from the Tet-response element (TRE). This vector is derived from pLNCX, a retroviral vector created using elements of Moloney murine leukemia virus (MoMuLV) and Moloney murine sarcoma virus (MoMuSV) as described. The TRE contains seven direct repeats of the tetO operator sequence, upstream of a minimal CMV promoter, which can be bound by the tTA and rtTA transactivators. The 5' viral LTR controls expression of the transcript that contains Y+ (the extended viral packaging signal) and the hygromycin resistance (Hygr) gene for antibiotic selection in mammalian cells.The TRE is derived vectors described previously. pRevTRE also includes the E. coli Ampr gene for antibiotic selection in bacteria.

 

The complete RevTet-Off and RevTet-On Systems also include the control vector pRevTRE-Luc, which was constructed by cloning the firefly luciferase gene into the HindIII/Cla I sites in the MCS of pRevTRE.

产品参数：

质粒类型：四环素调控系统

出品公司：Clontech

启动子：LTR

克隆方法：多克隆位点，限制性内切酶

载体大小：6487 bp 

载体抗性：Ampicillin (氨苄青霉素)

筛选标记：Hygromycin

备注：Tet-responsive retroviral expression.